Evidence map›Paper›PMID 41028477›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Adaptive Laboratory Evolution of Pichia pastoris by Serial Batch Cultivation.

Charles Moritz, Thomas Gassler, Martin Altvater, Diethard Mattanovich

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Charles MoritzDepartment of Biotechnology, Institute of Microbiology and Microbial Biotechnology, BOKU University, Vienna, Austria. charles.moritz@boku.ac.at.
Thomas GasslerInstitute of Microbiology, Department of Biology, ETH Zurich, Zurich, Switzerland.
Martin AltvaterDepartment of Biotechnology, Institute of Microbiology and Microbial Biotechnology, BOKU University, Vienna, Austria.
Diethard MattanovichDepartment of Biotechnology, Institute of Microbiology and Microbial Biotechnology, BOKU University, Vienna, Austria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adaptive laboratory evolution (ALE) is a research tool widely applied for the improvement and investigation of microorganisms. Multiple yeast species have been effectively targeted with this method, and Komagataella phaffii specifically is now well-suited for ALE experimentation due to advancements in its characterization and genetic manipulation. Here is presented a simple and generalizable protocol for the adaptation of K. phaffii towards growth on minimal media with methanol as the sole carbon source, along with suggestions on how this protocol may be adapted for other purposes. We give detailed steps for the repeated subculturing as well as recommended pre-experimental testing and post-experimental analysis. We also include notes and tips from hands-on experience with this methodology as applied specifically to K. phaffii.

Indexed as

Batch Cell Culture TechniquesPichiaSaccharomycetalesCulture MediaMethanolCulture MediaMethanolAdaptive laboratory evolutionKomagataella phaffiiPichia pastorisReverse engineeringSerial batch cultivationStrain engineering

Identifiers

PMID41028477

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.