Evidence map›Paper›PMID 41028462›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Pichia pastoris Expression Plasmid Assembly Using Type IIS Restriction Enzymes.

Alexandra Charlotte Ilse Grebe, Lia Jade Schallengruber, Carla Magdalena Aguilar Gomez, Lukas Rieder

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alexandra Charlotte Ilse Grebe *Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse, Graz, Austria.
Lia Jade Schallengruber *Institute of Molecular Biotechnology, Graz University of Technology, Petersgasse, Graz, Austria.
Carla Magdalena Aguilar GomezInstitute of Molecular Biotechnology, Graz University of Technology, Petersgasse, Graz, Austria.
Lukas RiederChristian Doppler Laboratory for Innovative Pichia pastoris Host and Vector Systems, Institute of Molecular Biotechnology, Graz University of Technology, Graz, Austria. rieder@tugraz.at.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A variety of cloning techniques are commonly used for the assembly of expression cassettes used for the transformation of Komagataella phaffii and, finally, the production of recombinant proteins. Restriction endonucleases are particularly useful due to their ability to recognize and cleave specific sequences in the DNA, allowing simple and reliable cloning strategies. However, the recognition sites of type II restriction enzymes leave a trace upon digestion since they cut within the palindromic recognition site. Hence, type IIS restriction enzymes that cleave outside of their recognition site became valuable tools for convenient and seamless single- and multi-gene cloning.

Indexed as

Cloning, MolecularDeoxyribonucleases, Type II Site-SpecificPlasmidsSaccharomycetalesGene ExpressionGenetic VectorsRecombinant ProteinsDeoxyribonucleases, Type II Site-SpecificRecombinant ProteinsKomagataella phaffiiPichia pastorisSeamless cloningType IIS restriction enzymes

Identifiers

PMID41028462

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.