Evidence map›Paper›PMID 41028458›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Establishing Reference Genes for Pichia pastoris Quantitative Real-Time Polymerase Chain Reaction Analyses.

Kai Büchner, Lana Vidal, Roland Kerpes, Thomas Becker

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kai BüchnerTechnical University of Munich, TUM School of Life Science, Chair of Brewing and Beverage Technology, Freising, Germany.
Lana Vidal
Roland KerpesTechnical University of Munich, TUM School of Life Science, Chair of Brewing and Beverage Technology, Freising, Germany. roland.kerpes@tum.de.
Thomas BeckerTechnical University of Munich, TUM School of Life Science, Chair of Brewing and Beverage Technology, Freising, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantitative real-time polymerase chain reaction (qPCR) can be used to investigate the relative expression of selected genes under different conditions; hence, it requires a robust endogenous reference consisting of various constitutive genes, also known as housekeeping genes. Thorough scrutiny of several genes under the specific conditions of the later experiments provides the most stably expressed candidate genes. The pairwise comparison and variance analysis of these candidate genes also yield the minimum number of reference genes for reliable RT-qPCR analysis. Therefore, this study selected three reference genes for the normalization and relative quantification of the gene expression of a Komagataella phaffii (previously classified as Pichia pastoris) wild-type strain using dextrose and methanol as different carbon sources.

Indexed as

Gene Expression ProfilingGenes, FungalPichiaReal-Time Polymerase Chain ReactionSaccharomycetalesGene Expression Regulation, FungalGlucoseMethanolReference StandardsGlucoseMethanolKomagataella phaffiiPichia pastorisReal-time quantitative PCR (RT-qPCR)Reference genesRelative quantification

Identifiers

PMID41028458

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.