Evidence map›Paper›PMID 41028439›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Characterization of Transgenic Rice Plants by Radioactive Northern Hybridization.

Rajasekaran Sripriya, Ajith Thaliyakkattil Girijan, Mahitha Koonjangadan Padinjhareveedu, Jasmine M Shah

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rajasekaran SripriyaDepartment of Zoology, St. Mary's College, Thoothukudi, Tamil Nadu, India.
Ajith Thaliyakkattil GirijanDepartment of Plant Science, Central University of Kerala, Kasaragod, Kerala, India.
Mahitha Koonjangadan PadinjhareveeduDepartment of Plant Science, Central University of Kerala, Kasaragod, Kerala, India.
Jasmine M ShahDepartment of Plant Science, Central University of Kerala, Kasaragod, Kerala, India. jasmine@cukerala.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Northern hybridization analysis of transgenic plants is done to confirm the presence of the transgene-derived transcript. Additionally, information on the size of the complete transcript and its relative expression can also be obtained by this technique. Presence of multiple forms (if any) of a transcript can also be visualized using this method. Radioactive labelling of DNA probe for Northern hybridization results in sensitive detection of the transcript. An elaborate protocol of Northern blotting and hybridization for detecting desired RNA from transgenic rice plants is described in this chapter.

Indexed as

Blotting, NorthernOryzaPlants, Genetically ModifiedRNA, PlantRNA, PlantNorthern blottingRadioactive Northern hybridizationRiceRNA

Identifiers

PMID41028439

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.