Evidence map›Paper›PMID 41028428›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Development of Transplastomic Tobacco Plants.

Kanakachari Mogilicherla, Janani Sree Sivakumar, Selvi Chellamuthu, Vasanth Krishnan, Dhandapani Gurusamy

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kanakachari MogilicherlaICAR-Indian Institute of Rice Research (IIRR), Rajendra Nagar, Hyderabad, Telangana, India.
Janani Sree SivakumarDepartment of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.
Selvi ChellamuthuDepartment of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Vasanth KrishnanDepartment of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.
Dhandapani GurusamyDepartment of Botany, Kongunadu Arts and Science College (Autonomous), Bharathiar University, Coimbatore, Tamil Nadu, India. gdhandapani@kongunaducollege.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chloroplasts, originating from ancestral cyanobacteria, are essential plastids involved in photosynthesis and other key metabolic processes, making them vital components of plant cells. Chloroplast transformation is a preferred alternative to nuclear transformation in plant genetic engineering due to its numerous advantages and positive outcomes. This chapter explores the available various chloroplast transformation vectors, cloning the gene of interest (GOI) in the chloroplast transformation vector with step-by-step protocol, and preparation of the vector for chloroplast transformation of tobacco using leaf disc explants through a biolistic method for efficient gene delivery. Additionally, it highlights how crucial some factors are for the effective integration and expression of the chloroplast genome by DNA-coated particle bombardment, including microcarrier type, DNA concentration, helium accelerating pressure, leaf layout and firing distance, and duration of pretreatment for explants. Further explored the generation of homoplasmic plants through re-regeneration with antibiotic selection confirmed correct cassette insertion and homoplasmy using PCR and Southern blot analyses and verified maternal inheritance of chloroplast-transferred genes by examining green and albino seedlings from reciprocal crosses between control and transplastomic tobacco plants.

Indexed as

ChloroplastsGenetic EngineeringNicotianaPlants, Genetically ModifiedBiolisticsGenetic VectorsGene Transfer TechniquesPlant LeavesTransformation, GeneticBiolistic transformationChloroplasts vectorMicrocarrierNicotiana tabacumSpectinomycin resistanceTransplastomic plants

Identifiers

PMID41028428

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.