ArticleMethods in molecular biology (Clifton, N.J.)2025
Agrobacterium-Mediated Genetic Transformation in Wheat.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Wheat (Triticum aestivum L.) is an important staple food crop that significantly impacts global food security. In terms of the need for an efficient genetic transformation protocol in major cereal crops, wheat comes immediately after rice to enhance their productivity level under varied global climatic conditions. Despite the availability of various Agrobacterium tumefaciens-based genetic transformation techniques for wheat crops, it remains the most challenging process and efficient transformation protocol continues to be a major hurdle in the development of genetically modified crops. Here in this study, we are describing the novel modified Agrobacterium tumefaciens-based in planta genetic transformation method which targets the 2-day-old apical meristematic germinated tissue as an explant for introgression of transgene. Germinated plants infected with Agrobacterium are screened for the presence of transgenes using PCR with gene-specific primers at the T
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