Evidence map›Paper›PMID 41028420›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Agrobacterium-Mediated Genetic Transformation and Genome Editing Using CRISPR-Cas9 Constructs in Rice.

M T Andrew-Peter-Leon, M Arumugam Pillai, Krish K Kumar, Velusamy Sumithra

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

M T Andrew-Peter-LeonS. Thangapazham Agricultural College (Affiliated to Tamil Nadu Agricultural University), Tenkasi, Tamil Nadu, India.ORCID https://orcid.org/0000-0002-1089-033X
M Arumugam PillaiDepartment of Plant Breeding and Genetics, V.O.C. Agricultural College and Research Institute, Tamil Nadu Agricultural University, Killikulam, Vallanadu, Tamil Nadu, India.ORCID https://orcid.org/0000-0002-4383-2574
Krish K KumarDepartment of Biotechnology, CPMB, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.ORCID https://orcid.org/0000-0002-9827-2295
Velusamy SumithraDepartment of Biotechnology, CPMB, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas)9 has become an essential tool in every molecular plant breeding laboratory. CRISPR-Cas9 causes highly precise knock-out mutations in plants that can be exploited in crop improvement programmes. However, poor response to Agrobacterium-mediated genetic transformation in recalcitrant rice genotypes is a major limiting factor. This protocol describes a detailed procedure for genome editing with CRISPR-Cas9 in recalcitrant rice genotypes that otherwise show a poor response to tissue culture. With this method, high transformation efficiency can be achieved in relatively a short period.

Indexed as

AgrobacteriumCRISPR-Cas SystemsGene EditingGenome, PlantOryzaTransformation, GeneticPlants, Genetically ModifiedAgrobacterium-mediated gene transferCRISPR-Cas9Recalcitrant rice

Identifiers

PMID41028420

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.