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ArticleMethods in molecular biology (Clifton, N.J.)2025

Agrobacterium-Mediated Genetic Transformation of Indica Rice.

Rajadurai Gothandaraman, Balaji Santhakumar, Vignesh Ponnurangan, Varanavasiappan Shanmugam, Krish K Kumar, Arul Loganathan, Kokiladevi Eswaran, Sudhakar Duraialagaraja

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Rajadurai GothandaramanDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Balaji SanthakumarDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Vignesh PonnuranganDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Varanavasiappan ShanmugamDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Krish K KumarDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Arul LoganathanDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
Kokiladevi EswaranDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India. kokiladevi@tnau.ac.in.
Sudhakar DuraialagarajaDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Functional genomics in rice has progressed significantly due to the availability of high-quality, well-annotated genome data and the characterization of numerous genes through genetic manipulation. Over the years, various protocols for genetic transformation in rice have been developed and refined. However, different rice cultivars respond differently to these protocols, there remains potential for further improvement and optimization. In this chapter, we have provided an efficient transformation protocol with critical points for the transformation of indica rice with rice immature embryos.

Indexed as

AgrobacteriumOryzaTransformation, GeneticPlants, Genetically ModifiedSeedsAgrobacterium-mediatedImmature embryosIndica riceProtocolRice transformation

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.