Expression Analysis of ROS-Related Genes During the Germination of Chickpea (Cicer Arietinum L.) Seeds.

Priyanka Babuta, Sanjib Bal Samant, Deepak Saini, Jagadis Gupta Kapuganti

Abstract read

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Priyanka BabutaNational Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.ORCID https://orcid.org/0000-0003-0598-2245

Sanjib Bal SamantNational Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.ORCID https://orcid.org/0009-0001-7575-1509

Deepak SainiNational Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.ORCID https://orcid.org/0000-0002-5353-7640

Jagadis Gupta KapugantiNational Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.ORCID https://orcid.org/0000-0002-7090-5097

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Seed germination is a critical physiological process that transforms a quiescent seed into a metabolically active seedling and is also a crucial factor in determining maximum crop production. This transition is influenced by various intrinsic and extrinsic factors. Interestingly, reactive oxygen species (ROS) plays an important role in breaking seed dormancy by oxidation of biomolecules, weakening of the testa and degradation of endosperm. Similarly, molecular internal oxygen is also considered vital for the transition of dormancy to seed germination. However, it is essential to establish a correlation between the internal oxygen and the generation of ROS during seed germination. This chapter details protocols for imaging internal oxygen concentrations using VisiSens and fluorescent detection of ROS using H

Indexed as

CicerGene Expression Regulation, PlantGerminationReactive Oxygen SpeciesSeedsOxygenPlant DormancyPlant ProteinsOxygenPlant ProteinsReactive Oxygen SpeciesChickpeaFluorescence imagingGene expressionInternal oxygenReactive oxygen speciesSeed germination

Identifiers

PMID41028384

DOI10.1007/978-1-4939-7292-0_4

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.