ArticleMethods in molecular biology (Clifton, N.J.)2026
Comprehensive Method for Soybean Cultivar Identification Using MGIseq, VCF2GENOME, and REST API.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This protocol provides a comprehensive method for identifying soybean (Glycine max) cultivars using a combination of advanced genomic and bioinformatic techniques. The process begins with DNA extraction from soybean leaf tissue, followed by MGIseq library construction and high-throughput sequencing. The resulting data undergoes rigorous quality control and preprocessing before being mapped to the soybean reference genome. Variant calling is then performed to generate a Variant Call Format (VCF) file, which is subsequently analyzed using the web application, VCF2GENOME and the REST API. The API facilitates the comparison of the sample's genetic profile with a curated database of known soybean cultivars, enabling accurate identification. This method is designed for researchers, breeders, and seed companies who require high-throughput, high-accuracy cultivar identification for various applications in soybean genetics, breeding, and seed quality control.
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