ArticleMethods in molecular biology (Clifton, N.J.)2026
Coupling Recombinant PhyB to Cell Culture Plates and Beads.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Opto-CD28-REACT: optogenetic co-stimulatory receptor activation on non-engineered human T cells.Frontiers in immunology · 2025Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optogenetics enables precise and reversible control of receptor activation using light-responsive proteins. In one approach, the first 651 amino acids of the photoreceptor phytochrome B (PhyB) of Arabidopsis thaliana are used as a light-responsive ligand for engineered receptors. These receptors are fused to the PhyB-interacting factor 6 (PIF) and bind to PhyB under red, but not under far-red light. The recombinant biotinylated PhyB ligand can be conjugated either to streptavidin-coated plates or to beads, enabling PIF-fused receptor activation with high valency. This method provides high spatiotemporal precision, offering potential for studying receptor signaling in two-dimensional cell-free interactions. Here, we provide protocols to couple recombinant PhyB to plastic plates or polystyrene beads and validate the coupling using light-dependent stimulation of green fluorescent protein (GFP)-PIF-fused T-cell receptors (TCRs) in primary human T cells.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.