ArticleMethods in molecular biology (Clifton, N.J.)2026
Opto-APCs: Displaying Phytochrome B on the Surface of Living Cells for Optogenetic Studies.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Opto-CD28-REACT: optogenetic co-stimulatory receptor activation on non-engineered human T cells.Frontiers in immunology · 2025Article
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Authors and funding
4 authors.
Funding
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Abstract
Interactions between proteins on the surface of two cells are pivotal in determining cell fate. These might be receptor-ligand pairs or adhesion molecules. Mechanistic and functional details of these interactions can be studied by optogenetics using parts of the photoreceptor phytochrome B (PhyB) of Arabidopsis thaliana. In these cases, PhyB serves as a membrane-bound ligand for engineered receptors or adhesion molecules. Here, we provide a protocol to generate cells that display PhyB as a membrane-bound protein on their surface to serve as the ligand. This is done by expressing a transmembrane SpyCatcher protein on the cells and covalently coupling purified recombinant PhyB-SpyTag to these cells. Coming from immunology, we call the PhyB-coupled cells opto-antigen-presenting cells (opto-APCs).
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