Evidence map›Paper›PMID 41028350›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Analyzing In Vitro Autokinase Activity of Microbial Phytochromes Under Well-Defined Light Conditions.

Christina Huber, Merle Strack, Rolf Diller, Nicole Frankenberg-Dinkel

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Christina HuberDepartment of Biology, Microbiology, RPTU University Kaiserslautern-Landau, Kaiserslautern, Germany.
Merle StrackDepartment of Physics, Biophysics, RPTU University Kaiserslautern-Landau, Kaiserslautern, Germany.
Rolf DillerDepartment of Physics, Biophysics, RPTU University Kaiserslautern-Landau, Kaiserslautern, Germany.
Nicole Frankenberg-DinkelDepartment of Biology, Microbiology, RPTU University Kaiserslautern-Landau, Kaiserslautern, Germany. nicole.frankenberg@rptu.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbial phytochromes primarily function as sensor histidine kinases in two-component regulatory systems, with light-triggered conformational changes driving varying autokinase outputs. Analyzing these responses under different light conditions is key to understanding phytochrome functions in host organisms.Here, we introduce a novel method for providing quantitative correlation between kinase activity and calculated Pr/Pfr fractions as determined by spectroscopic measurements. The approach includes data analysis for calculating Pr/Pfr content using UV/Vis absorption spectroscopy. It also introduces a Kinase Illumination Module (KIM) that creates a controlled light environment during autokinase assays, replicating conditions from UV/Vis spectroscopy to ensure consistent photostationary states. The method provides a versatile platform for investigating the dynamics of phytochrome signaling pathways, which is applicable to a wide range of experimental conditions.

Indexed as

Bacterial ProteinsHistidine KinaseLightPhytochromeSignal TransductionBacterial ProteinsHistidine KinasePhytochromeAutokinase assayDefined Pr/Pfr-ratiosLight-dependent kinaseLight-regulated kinaseLight sensingPhotoreceptionSignal transductionSteady-state conditions

Identifiers

PMID41028350

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.