Evidence map›Paper›PMID 41028344›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Spectroscopic Analyses of Photoconversion in Phytochromes.

Chengwei Yi, Stefanie S M Meier, Maria Kehr, Andreas Möglich

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chengwei Yi *Department of Biochemistry, University of Bayreuth, Bayreuth, Germany.ORCID https://orcid.org/0000-0002-2167-4536
Stefanie S M Meier *Department of Biochemistry, University of Bayreuth, Bayreuth, Germany.ORCID https://orcid.org/0009-0002-6028-5223
Maria Kehr *Department of Biochemistry, University of Bayreuth, Bayreuth, Germany.ORCID https://orcid.org/0000-0001-6690-9696
Andreas MöglichDepartment of Biochemistry, University of Bayreuth, Bayreuth, Germany. andreas.moeglich@uni-bayreuth.de.ORCID https://orcid.org/0000-0002-7382-2772

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phytochrome photoreceptors integrate red and far-red light to inform vital adaptations of physiology, development, and lifestyle in manifold plants, bacteria, and fungi. Once covalently assembled with bilin chromophores, phytochromes traverse their metastable Pr and Pfr states depending on light quality and intensity. This chapter provides protocols for the analyses of phytochromes by absorption spectroscopy to determine their molar extinction coefficients, their photostationary state assumed under constant illumination, and their quantum yields for the light-driven Pr ⇄ Pfr interconversion. Knowledge of these parameters pertains to the natural role of phytochromes and their deployment in optogenetics and biotechnology.

Indexed as

PhytochromeSpectrum AnalysisLightPhytochromeBilinChromophoreOptogeneticsPhotobiologyPhotoreceptorPhytochromeQuantum yieldSpectroscopy

Identifiers

PMID41028344

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.