Evidence map›Paper›PMID 41028340›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Characterizing Phytochrome Photochemistry Using UV-Visible Spectroscopy in Host Cell Extracts and Purified Protein.

Jimena Rinaldi, Lisandro H Otero, Hernán R Bonomi

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jimena RinaldiInstituto de Nanosistemas, Universidad Nacional de San Martín, Campus Miguelete, Buenos Aires, Argentina. jrinaldi@unsam.edu.ar.
Lisandro H OteroDepartamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Instituto de Biotecnología Ambiental y Salud, CONICET, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Hernán R BonomiGenerate Biomedicines, Somerville, MA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phytochromes are red and far-red photoreceptors widespread among life, which bind a bilin chromophore molecule. They photoswitch between two main or parent photostates: the Pr state, which absorbs red light, and the Pfr state, which absorbs far-red light. Apart from the photoconversion, there is another process called dark conversion. The phytochrome photocycle can be easily followed by ultraviolet (UV)-visible or visible spectroscopy. Through the acquisition and the later data analysis of serial UV-visible absorption spectra in either unpurified or purified protein samples, many important aspects of the phytochrome photocycle can be learned.

Indexed as

PhotochemistryPhytochromePhotochemical ProcessesSpectrophotometry, UltravioletUltraviolet RaysPhytochromeBacterial crude extractsBilin chromophoreBiliverdin IXα chromophorePhotocycle dynamicsPhotocycle kinetics modelingPhytochromesPr/Pfr abundance analysisPurified protein samplesRed/far-red light photoreceptorsSpectral decompositionUV–visible absorption spectroscopy

Identifiers

PMID41028340

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.