ArticleMethods in molecular biology (Clifton, N.J.)2026
Overcoming the Challenges of Producing Plant Phytochromes in Escherichia coli.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biochemical and structural studies of plant phytochromes have been limited until recently by the difficulty of producing them in large amounts and as chromophore-bound, photochemically competent holoproteins in bacterial expression systems. This chapter provides protocols to achieve high-level production of the photosensory modules of plant phytochrome B in Escherichia coli. Protocols for codon optimization, designing multiple constructs, coexpression of the bilin chromophore synthesizing enzymes, and optimizing expression vectors, expression strains, and growth conditions are also included. These protocols can be easily adopted to overcome frequently encountered problems during heterologous production of eukaryotic phytochromes in Escherichia coli.
Indexed as
Identifiers
41028339What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.