ArticleMethods in molecular biology (Clifton, N.J.)2026
Discovery and Engineering of Unique Cyanobacteriochrome GAF Domains.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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7 authors.
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Abstract
The linear tetrapyrrole-binding cyanobacteriochrome (CBCR) photoreceptors have been extensively analyzed so far and found to be highly diverse in spectral properties. Here, we describe the methods to search novel CBCR molecules from the database, perform site-saturation random mutagenesis and colony-based protein expression, and express the CBCR molecules with various chromophore synthetic systems. These methods will contribute to further discovery and engineering of the CBCR molecules.
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