Evidence map›Paper›PMID 41028284›Full record

ArticleCommunications biology2025

CCAAT/enhancer binding protein beta (C/EBPβ) regulates the formation of the ovarian reserve.

Xiaoyuan Zhang, Yue Zeng, Zhanzhong Qiao, Kexin Qin, Pei Li, Jiamao Yan, Teng Zhang, Yang Zhou, Junjie Wang, Wei Shen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiaoyuan Zhang *College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.ORCID http://orcid.org/0000-0002-5830-6606
Yue Zeng *College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Zhanzhong QiaoCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Kexin QinCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Pei LiCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Jiamao YanCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
Teng ZhangState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock (R2BGL), College of Life Sciences, Inner Mongolia University, Hohhot, China.ORCID http://orcid.org/0000-0001-7773-2515
Yang ZhouState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock (R2BGL), College of Life Sciences, Inner Mongolia University, Hohhot, China. zhouyang106@126.com.ORCID http://orcid.org/0000-0003-1768-0244
Junjie WangCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China. 202101017@qau.edu.cn.ORCID http://orcid.org/0000-0002-7145-5702
Wei ShenCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China. wshen@qau.edu.cn.ORCID http://orcid.org/0000-0001-6112-2501

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32270903
6 · The paper itself

Abstract

The primordial follicle (PF) pool (also known as ovarian reserve) is the only source of functional eggs during the reproductive lifespan, which formed at embryonic stage in most female mammals and is no longer renewed. CCAAT/enhancer binding protein beta (C/EBPβ) is a transcription factor (TF) and widely expressed in adult ovarian granulosa cells, but its role in the early ovarian development remains unclear. Here, we showed that C/EBPβ was enhanced during PF formation process in mice, and underwent nuclear translocation in germ cells along with the PF assembly (PFA) process. Importantly, the in vitro knockdown of C/EBPβ could inhibit the related proteins expression, resulting in the obstruction of PF formation. Mechanistically, the chromatin accessibility analysis revealed that C/EBPβ binds to the promoter region of the histone acetylase encoding gene Ep300 to promotes its expression, and enhance neurotrophic tyrosine receptor kinase (NTRK) signaling (required for PF formation) by maintaining chromatin accessibility of Furin promoter region. Interestingly, our results verified that the nuclear translocation of C/EBPβ is regulated by its phosphorylation level, and Lamin B1 acts as a "gatekeeper" molecule for the process. In summary, this report suggests that C/EBPβ is a key regulator for the establishment of ovarian reserve in mice.

Indexed as

CCAAT-Enhancer-Binding Protein-betaOvarian FollicleOvarian ReserveAnimalsFemaleGranulosa CellsMiceMice, Inbred C57BLCCAAT-Enhancer-Binding Protein-betaCebpb protein, mouse

Identifiers

PMID41028284
PMCPMC12484758

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.