Evidence map›Paper›PMID 41028059›Full record

ArticleScientific reports2025

Season affects sperm quality, seminal extracellular vesicles, and antioxidant-related genes in Egyptian Buffalo bulls.

Abdallah M Shahat, Mohamed O Taqi, Heba F Hozyen, Samaa M Galal, Abdel Aziz M Sakr, Sameh M Shedeed, Karima Gh M Mahmoud, Amal R Abd El Hameed, Mohamed F Hashem, John P Kastelic and 1 more

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Abdallah M Shahat *Department of Theriogenology, Faculty of Veterinary Medicine, Giza, Egypt. abdallah.mohamed@fvsu.edu.ORCID http://orcid.org/0000-0001-5519-0743
Mohamed O Taqi *Central Laboratory for Agricultural Climate, Agricultural Research Center, Ministry of Agriculture and Land Reclamation, Dokki, Giza, Egypt.
Heba F Hozyen *Department of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
Samaa M GalalDepartment of Theriogenology, Faculty of Veterinary Medicine, Giza, Egypt.
Abdel Aziz M SakrDepartment of Biotechnology Research, Animal Production Research Institute (APRI), Agricultural Research Center, Giza, Egypt.
Sameh M ShedeedDepartment of Biotechnology Research, Animal Production Research Institute (APRI), Agricultural Research Center, Giza, Egypt.
Karima Gh M MahmoudDepartment of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
Amal R Abd El HameedDepartment of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
Mohamed F HashemDepartment of Theriogenology, Faculty of Veterinary Medicine, Giza, Egypt.
John P KastelicFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, T2N 4N1, Canada.
Sally IbrahimDepartment of Animal Reproduction and A.I, Veterinary Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt. sally_rashad2004@yahoo.com.ORCID http://orcid.org/0000-0003-2098-6463

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Global climate change threatens livestock production, especially in regions where heat stress diminishes fertility. Extracellular vesicles (EVs), key players in biological processes like intercellular communication and oxidative stress regulation, are crucial in reproductive biology. The objective was to determine seasonal impacts on semen quality, oxidative stress, and antioxidant enzyme activity in Egyptian buffalo bulls, focusing on differences in EV content between bulls having high- or low-quality sperm (HQS and LQS, respectively), based on sperm motility and morphology. Semen samples were collected by artificial vagina in summer and winter. Oxidative stress markers, such as malondialdehyde (MDA), and antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]) were measured in seminal plasma. Seminal plasma EVs (SP-EVs) were isolated and characterized by size, concentration, and surface markers (CD9 and CD63). Additionally, gene expression of antioxidant and apoptotic markers was analyzed using qRT-PCR. Semen quality peaked in the winter, with HQS exhibiting superior total motility (winter total motility: 79.4 ± 0.65% vs. summer: 69.9 ± 0.65%) and morphology (winter normal morphology: 75.5 ± 0.87% vs. summer: 71.3 ± 0.87%). Furthermore, LQS semen had higher MDA concentrations (summer: 1.31 ± 1.67 nmol/ml; winter: 2.62 ± 1.21 nmol/ml), indicating greater oxidative stress, whereas HQS exhibited elevated antioxidant enzyme activity, especially in summer (SOD: 292.0 ± 3.93 U/ml; CAT: 949.7 ± 15.23 U/ml; GPx: 77.7 ± 2.15 mU/ml; TAC: 9.83 ± 4.75 mM/L). Notably, SP-EVs from LQS were larger and had increased CD9 and CD63 expression, suggesting their association with oxidative damage. Conversely, SP-EVs from HQS were smaller, had lower CD9 and CD63 expression, and had upregulated (p ≤ 0.001) antioxidant genes (SOD, NFE2L2) and downregulated (p ≤ 0.001) apoptotic markers (CASP3). Expression of DNMT1 and ATF6 mRNA were elevated (p ≤ 0.001) in HQS sperm and SP-EVs, especially in summer, indicating a seasonal effect on epigenetic regulation and stress response in reproductive health. These findings highlighted the potential of SP-EVs as biomarkers for semen quality, their role in protecting sperm functions under seasonal stress, and epigenetic mechanisms, providing critical insights for improving the reproductive performance of buffalo bulls under climate-induced stress.

Indexed as

AntioxidantsBuffaloesExtracellular VesiclesSeasonsSemenSemen AnalysisSpermatozoaAnimalsCatalaseEgyptMaleOxidative StressSperm MotilitySuperoxide DismutaseAntioxidantsCatalaseSuperoxide DismutaseBuffalo bullsExtracellular vesiclesGene expressionOxidative markerSemen qualitySeminal plasma

Identifiers

PMID41028059
PMCPMC12485197

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.