Evidence map›Paper›PMID 41027914›Full record

ArticleNature communications2025

Induction of experimental cell division to generate cells with reduced chromosome ploidy.

Nuria Marti Gutierrez, Aleksei Mikhalchenko, Maria Shishimorova, Daniel Frana, Crystal Van Dyken, Ying Li, Hong Ma, Amy Koski, Dan Liang, Sang-Goo Lee and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Ovarian ageing: from biological constraint to emerging therapeutic frontier.Journal of assisted reproduction and genetics · 2026
    Review
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  4. What a (re)productive year it has been!Journal of assisted reproduction and genetics · 2026
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Nuria Marti GutierrezCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Aleksei MikhalchenkoCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Maria ShishimorovaCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Daniel FranaCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Crystal Van DykenCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.ORCID http://orcid.org/0000-0002-3293-9333
Ying LiCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Hong MaCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Amy KoskiCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.ORCID http://orcid.org/0000-0003-1918-528X
Dan LiangCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.ORCID http://orcid.org/0000-0003-2404-3075
Sang-Goo LeeCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Daniel EybergCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Zahra SafaeiCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA.
Eunju KangDepartment of Biomedical Science and CHA Advanced Research Institute, CHA University, Gyeonggi-do, Republic of Korea.
Yeonmi LeeDepartment of Biomedical Science and CHA Advanced Research Institute, CHA University, Gyeonggi-do, Republic of Korea.
Thomas O'LearyDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR, 97239, USA.
David LeeDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR, 97239, USA.
Sacha KriegDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR, 97239, USA.
Diana WuDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR, 97239, USA.
Elizabeth RubinDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR, 97239, USA.
Paula AmatoCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA. amatop@ohsu.edu.
Shoukhrat MitalipovCenter for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, 97239, USA. shoukhrat@gmail.com.ORCID http://orcid.org/0000-0003-0937-6146

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Somatic cell nuclear transfer (SCNT) enables the direct reprogramming of somatic cells into functional oocytes, albeit with a diploid genome. To address ploidy reduction, we investigated an experimental reductive cell division process, termed mitomeiosis, wherein non-replicated (2n2c) somatic genomes are prematurely forced to divide following transplantation into the metaphase cytoplasm of enucleated human oocytes. However, despite fertilization with sperm, SCNT oocytes remained arrested at the metaphase stage, indicating activation failure. Artificial activation using a selective cyclin-dependent kinase inhibitor successfully bypassed this arrest, inducing the segregation of somatic chromosomes into a zygotic pronucleus and a polar body. Comprehensive chromosome tracing via sequencing revealed that homologous chromosome segregation occurred randomly and without crossover recombination. Nonetheless, an average of 23 somatic chromosomes were retained within the zygote, demonstrating the feasibility of experimentally halving the diploid chromosome set. Fertilized human SCNT oocytes progressed through normal embryonic cell divisions, ultimately developing into embryos with integrated somatic and sperm-derived chromosomes. While our study demonstrates the potential of mitomeiosis for in vitro gametogenesis, at this stage it remains just a proof of concept and further research is required to ensure efficacy and safety before future clinical applications.

Indexed as

Cell DivisionNuclear Transfer TechniquesOocytesPloidiesChromosome SegregationFemaleHumansMaleSpermatozoaZygote

Identifiers

PMID41027914
PMCPMC12485136

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.