ArticleNature communications2025
Prominent involvement of acetylcholine dynamics in stable olfactory representation across the Drosophila brain.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Advances in Targeting Central Cholinergic Dysfunction for Neurodegenerative Diseases: From Pharmacotherapy to Neuromodulation.CNS neuroscience & therapeutics · 2026Review
- Constructing mesoscale functionomics by neural dynamics subspace clustering.National science review · 2026Article
- CELLECT: contrastive embedding learning for large-scale efficient cell tracking.Nature methods · 2025Article
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
Despite the vital role of neuromodulators and neurotransmitters in the neural system, their spatiotemporal correlation with neuronal activities across multiple brain regions remain unclear. Here, we employed two-photon synthetic aperture microscopy (2pSAM) and neurochemical indicators to simultaneously record calcium and acetylcholine (ACh)/5-HT dynamics across multiple regions of the Drosophila brain over 2 h. Presenting 3 different odors across multiple trials, our analyses revealed signal-specific differences in responsiveness, functional connectivity, and odor classification accuracy across the brain. We further constructed low-dimensional manifolds to characterize the global odor-related dynamics. Incorporating both calcium and ACh signals enhanced odor classification accuracy in the global low-dimensional manifold and in specific brain regions where their functional connectivity network features exhibited complementary patterns. Moreover, ACh dynamics demonstrated relatively stable temporal characteristics compared to calcium and 5-HT. These results suggest the potential contribution of ACh to consistent odor representations and illustrate the utility of multi-signal imaging in studying neural computation.
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Registered trials
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