Evidence map›Paper›PMID 41027712›Full record

ArticleRNA (New York, N.Y.)2025

Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa).

Prasoon K Thakur, Anzhelika Butenko, Filip Karásek, Michaela Svobodová, Drahomíra Faktorová, Hana Pavlisková, Vladimir Varga, Aleš Horák, Julius Lukeš, David Staněk

Abstract read
In one paragraph

Article in RNA (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Prasoon K ThakurInstitute of Molecular Genetics, Czech Academy of Sciences, Prague, 142 20, Czech Republic.ORCID 0000-0003-2217-9008
Anzhelika ButenkoInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), 370 05, Czech Republic.ORCID 0000-0001-8685-2404
Filip KarásekInstitute of Molecular Genetics, Czech Academy of Sciences, Prague, 142 20, Czech Republic.
Michaela SvobodováInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), 370 05, Czech Republic.
Drahomíra FaktorováInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), 370 05, Czech Republic.ORCID 0000-0001-9623-2233
Hana PavliskováInstitute of Molecular Genetics, Czech Academy of Sciences, Prague, 142 20, Czech Republic.
Vladimir VargaInstitute of Molecular Genetics, Czech Academy of Sciences, Prague, 142 20, Czech Republic.ORCID 0000-0002-0384-1281
Aleš HorákInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), 370 05, Czech Republic.ORCID 0000-0002-3364-7383
Julius LukešInstitute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), 370 05, Czech Republic.ORCID 0000-0002-0578-6618
David StaněkInstitute of Molecular Genetics, Czech Academy of Sciences, Prague, 142 20, Czech Republic stanek@img.cas.cz.ORCID 0000-0002-5865-175X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Noncoding introns are a unifying feature of protein-coding genes in virtually all extant eukaryotes, with most lineages following the canonical intron structure. However, euglenozoans, unicellular flagellates that include free-living euglenids, human pathogenic kinetoplastids, and highly diverse and abundant marine diplonemids, are a notable exception. Euglenozoan genomes range from extremely intron-poor kinetoplastids to euglenid genomes containing both canonical and noncanonical introns. Here, we present a comprehensive analysis of splice sites and spliceosomal components in six species of understudied diplonemids. All diplonemids examined contain a nearly complete set of spliceosomal snRNP components, indicating the presence of a functional U2-type spliceosome. However, the majority of introns in the hemistasiid diplonemids

Indexed as

EuglenozoaGenes, ProtozoanIntronsRNA, ProtozoanRNA Splice SitesBase SequenceGenetic VariationRibonucleoproteins, Small NuclearRNA, Small NuclearRNA SplicingSpliceosomesRibonucleoproteins, Small NuclearRNA, ProtozoanRNA, Small NuclearRNA Splice Sitesdiplonemidseuglenozoansnoncanonical intronssnRNPspliceosome

Identifiers

PMID41027712
PMCPMC12621601

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.