Evidence map›Paper›PMID 41026775›Full record

ArticleCancer reports (Hoboken, N.J.)2025

Notch1 Mutation Represents a Potential Therapeutic Target to Enhance Immune Recognition in Oral Squamous Cell Carcinoma.

Takahiro Iwamoto, Kazuhiro Ogi, Takafumi Nakagaki, Takashi Sasaya, Sho Miyamoto, Koyo Nishiyama, Kenta Sasaki, Shintaro Sugita, Yasushi Sasaki, Akihiro Miyazaki

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PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer reports (Hoboken, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Takahiro IwamotoDepartment of Oral Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.ORCID 0009-0004-9485-1822
Kazuhiro OgiDepartment of Oral Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.ORCID 0000-0002-0104-9223
Takafumi NakagakiDepartment of Oral Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.
Takashi SasayaDepartment of Oral Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.
Sho MiyamotoDepartment of Oral Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.ORCID 0000-0001-9573-1161
Koyo NishiyamaDepartment of Oral Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.
Kenta SasakiDepartment of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.ORCID 0000-0001-8070-3045
Shintaro SugitaDepartment of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Yasushi SasakiBiology Division, Department of Liberal Arts and Sciences, Center for Medical Education, Sapporo Medical University, Sapporo, Japan.ORCID 0000-0002-3500-8059
Akihiro MiyazakiDepartment of Oral Surgery, Sapporo Medical University School of Medicine, Sapporo, Japan.ORCID 0000-0003-3290-6205

Funding

Japan Society for the Promotion of Science JSPS KAKENHI 22K10197Japan Society for the Promotion of Science JSPS KAKENHI 23K27794Japan Society for the Promotion of Science JSPSKAKENHI25K13171
6 · The paper itself

Abstract

backgroundNotch1, a tumor suppressor gene, is one of the most frequently mutated genes in head and neck squamous cell carcinoma (HNSCC). Therefore, it is clinically important to investigate the effects of Notch1 mutations on antitumor immunity in oral squamous cell carcinoma (OSCC), a subset of HNSCC.

aimsThis study investigated Notch1 mutations and the expression of immune-related proteins. We also examined the influence of Notch1 mutations on the immune microenvironment using a public database. METHODS AND

resultsWe examined the expression of Notch1-4 in OSCC cell lines using qPCR. After Notch1 knockdown, OSCC cell proliferation and migration were analyzed using CCK8 and wound healing assays, respectively. Localization of programmed cell death ligand 1 (PD-L1) was assessed by western blot and flow cytometry, while PD-L1 expression was evaluated by western blot. In the somatic mutation analysis of 47 OSCC patients, the relationship between tumor-infiltrating CD8

conclusionThese findings highlight Notch1 mutation as a potential therapeutic target for immune recognition in OSCC.

Indexed as

Carcinoma, Squamous CellMouth NeoplasmsReceptor, Notch1Squamous Cell Carcinoma of Head and NeckB7-H1 AntigenCD8-Positive T-LymphocytesCell Line, TumorCell MovementCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansLymphocytes, Tumor-InfiltratingMaleMiddle AgedMutationB7-H1 AntigenCD274 protein, humanNOTCH1 protein, humanReceptor, Notch1CD8+ T cellsimmunotherapyNotch1 mutationoral squamous cell carcinomaprogrammed cell deathtumor microenvironment

Identifiers

PMID41026775
PMCPMC12483097

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.