Evidence map›Paper›PMID 41026425›Full record

ArticleJournal of assisted reproduction and genetics2025

Pre-incubation with levocarnitine alleviates vitrification and thawing damage in mouse oocytes.

Shutian Jiang, Jing Zhang, Xueyi Jiang, Yu Tao, Jing Yang, Kanru Zhao, Nannan Li, Luping Feng, Haoran Shen, Yao Wang

Erratum issuedAbstract read
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In one paragraph

Article in Journal of assisted reproduction and genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Shutian Jiang *Department of Assisted Reproduction, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.ORCID http://orcid.org/0000-0003-3459-7442
Jing Zhang *Department of Assisted Reproduction, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Xueyi Jiang *Department of Assisted Reproduction, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Yu TaoDepartment of Assisted Reproduction, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Jing YangDepartment of Assisted Reproduction, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Kanru ZhaoDepartment of Assisted Reproduction, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Nannan LiDepartment of Anatomy and Physiology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Luping FengCenter for Brain Science, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Haoran ShenHospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China. shenhaoran@fudan.edu.cn.
Yao WangDepartment of Assisted Reproduction, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. drwangyao@163.com.

Funding

National Natural Science Foundation of China 82201886Natural Science Foundation of Shanghai Municipality 24ZR1443500
6 · The paper itself

Abstract

backgroundVitrification of mammalian oocytes is an important means of preserving female reproductive capacity. However, existing technical solutions cannot guarantee recovery rate, fertilization, and developmental potential of vitrified oocytes. Recognized consequences include energy consumption during vitrification and thawing processes, and damage to organelles caused by the formation of ice crystals. In this study, we explore whether incubating with levocarnitine (LC) before vitrification can improve its warming outcomes.

resultsSupplementing 2 mg/mL LC can significantly improved recovery rate of warmed oocytes (p < 0.01) and shortened morphological recovery time (p < 0.001). Gene difference analysis showed that supplementing LC altered gene expression related to energy metabolism, autophagy, and cytoskeleton. Further experimental testing showed that supplementing LC increased adenosine triphosphate (ATP) levels (p < 0.05), decreased reactive oxygen species (ROS) levels (p < 0.001), and maintained the stability of the spindle chromosome structure. On this basis, both lipid oxidation (p < 0.01) and autophagy levels (p < 0.0001) in oocytes decreased, and the quality of embryos developed from warmed oocytes was improved (p < 0.05).

conclusionSupplementation of LC to mouse oocytes before vitrification can improve their warming outcomes, which is related to the changes in lipid droplet distribution in oocytes caused by LC by improving lipid metabolism.

Indexed as

CryopreservationOocytesVitrificationAnimalsAutophagyCryoprotective AgentsEnergy MetabolismFemaleFertilization in VitroMiceReactive Oxygen SpeciesCryoprotective AgentsReactive Oxygen SpeciesApoptosisGenesLevocarnitineOocyte cryopreservationOxidation–reduction

Identifiers

PMID41026425
PMCPMC12705919

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.