Mycoplasma leachii strain PG50 shows reduced adherence and invasion compared to M. bovis strains 428E and PG45.

Daniel W Nielsen, Samantha J Hau, David J Holthausen, Rohana P Dassanayake, Bryan S Kaplan

Abstract readComparative Study

In one paragraph

Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Daniel W NielsenRuminant Diseases and Immunology Research Unit, USDA-ARS National Animal Disease Center, Ames, IA, USA. Daniel.nielsen@usda.gov.

Samantha J HauVirus and Prion Research Unit, USDA-ARS National Animal Disease Center, Ames, IA, USA.

David J HolthausenRuminant Diseases and Immunology Research Unit, USDA-ARS National Animal Disease Center, Ames, IA, USA.

Rohana P DassanayakeRuminant Diseases and Immunology Research Unit, USDA-ARS National Animal Disease Center, Ames, IA, USA.

Bryan S KaplanRuminant Diseases and Immunology Research Unit, USDA-ARS National Animal Disease Center, Ames, IA, USA.

Funding

Agricultural Research Service 5030-32000-236-000-D

6 · The paper itself

Abstract

Mycoplasma leachii is a member of the "Mycoplasma mycoides cluster", which includes the causative agent of contagious bovine pleuropneumonia (Mycoplasma mycoides subsp. mycoides). Mycoplasma leachii has been associated with severe outbreaks of polyarthritis, mastitis, and abortion in dairy cattle in Australia, Argentina, and China. As M. leachii has veterinary implications and is related to other foreign animal diseases of economic importance, we sought to investigate differences in adherence to and invasion of different bovine cells between M. leachii type strain PG50 and M. bovis strains (type strain PG45 and field strain 428E). Specifically, we tested the strains with Madin-Darby bovine kidney (MDBK), bovine turbinate (BTu), and the carticular progenitor 5 (CP5) cells for 2 and 24 h. Additionally, we tested the MDBK and BTu cells with both non-cytopathic bovine viral diarrhea virus (BVDV-1b) positive and BVDV negative fetal bovine serum. Mycoplasma leachii strain PG50 adhered and invaded less efficiently than M. bovis strains 428E and PG45 with a log

Indexed as

Bacterial AdhesionCattle DiseasesMycoplasmaMycoplasma bovisMycoplasma InfectionsAnimalsCattleCell LineAdherenceBVDVCell cultureMycoplasma bovisMycoplasma leachii

Identifiers

PMID41026261

DOI10.1007/s11259-025-10901-x

PMCPMC12484092

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.