ArticleVeterinary medicine and science2025
Serological and Molecular Detection of Toxoplasma gondii Among Slaughtered Domestic Ruminants in Gondar Town, Northwest Ethiopia.
Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Serological and Molecular Detection of Toxoplasma gondii Among Slaughtered Domestic Ruminants in Gondar Town, Northwest Ethiopia.Veterinary medicine and science · 2025Article
- Serological and virological characterization of hepatitis B surface antigen-positive patients with or without Helicobacter pylori co-infection at the university of Gondar comprehensive specialized hospital, Northwest Ethiopia.BMC infectious diseases · 2025Article
- Clinical and biochemical characterization of hepatitis B surface antigen-positive patients with or withoutFrontiers in immunology · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundToxoplasma gondii is a globally prevalent zoonotic protozoan parasite that forms cysts and infects both animals and humans. Although there are reports on its seroprevalence, no comparative studies have validated the presence of this parasite in Ethiopian food animals.
objectivesThis study assessed and compared serological and molecular techniques for the detection of T. gondii in slaughtered domestic ruminants in the study area.
methodsA cross-sectional study was conducted from September 2019 to October 2020 by collecting 320 blood and corresponding tissue samples from purposively selected animals. The presence of this disease was identified using the latex agglutination test (LAT) and nested polymerase chain reaction (nPCR). SPSS version 25 with chi-square and logistic regression analyses was used to determine the relationship between associated risk factors and seropositivity of this disease.
findingsThe overall findings on the presence of this infection among slaughtered animals showed positivity rates of 180 (56.2%) through latex ATs, 68 (21.2%) in the first PCR run and 34 (10.6%) in the second PCR run. In latex ATs, positivity rates were recorded as 62% (62/100) in sheep, 52.7% (58/110) in goats and 54.5% (60/110) in cattle. The first PCR amplification detected positive cases in 34% (34/100) of sheep, 21.8% (24/110) of goats and 9.1% (10/110) of cattle. The second PCR amplification identified positivity in 20% (20/100) of sheep and 12.5% (14/110) of goats, whereas no positive cases were found in cattle. Seropositivity showed a significant association with sex and age in sheep and goats, origin in goats and breed and age in cattle (p value ≤ 0.05). A slight level of agreement was observed between latex agglutination and the first PCR test (Kappa = 0.230), whereas a fair agreement was noted between first PCR and nPCR (K = 0.338). Overall, nPCR demonstrated higher specificity (83.9%) compared to first PCR and the latex AT for detecting this disease.
conclusionsThus, comparative detection confirms its presence in slaughtered food animals, aiding in early effective prevention and control for food consumption. Further research on pathogen genotyping is strongly recommended.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.