Evidence map›Paper›PMID 41025540›Full record

ArticleInternational forum of allergy & rhinology2026

Patient-Specific Coculture of S. aureus and P. aeruginosa Enhances Epithelial Barrier Disruption and Virulence in CRS.

Xiaohan Sun MMed, Mahnaz Ramezanpour, Jordan Hall, Emma Barry, Alkis J Psaltis, Peter-John Wormald, Sarah Vreugde

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Article in International forum of allergy & rhinology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiaohan Sun MMedDepartment of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.ORCID https://orcid.org/0009-0006-9018-8300
Mahnaz RamezanpourDepartment of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.ORCID https://orcid.org/0000-0001-6218-2071
Jordan HallDepartment of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.
Emma BarryDepartment of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.
Alkis J PsaltisDepartment of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.
Peter-John WormaldDepartment of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.
Sarah VreugdeDepartment of Surgery-Otolaryngology, Head and Neck Surgery, Central Adelaide Local Health Network, Woodville, South Australia, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChronic rhinosinusitis (CRS) is a chronic inflammatory disease that is associated with polymicrobial infections, often involving S. aureus and P. aeruginosa. It is unclear whether the polymicrobial context plays a role in exacerbating epithelial damage, inflammation, and resistance to therapy.

methodsS. aureus and P. aeruginosa (n = 3 each) biofilms were established in a Transwell system, followed by the extraction of P. aeruginosa conditioned media and application to an air-liquid interface (ALI) model of human nasal epithelial cells (HNECs). Transepithelial electrical resistance (TEER) and FITC dextran paracellular permeability tests evaluated the epithelial integrity. Colony-forming unit (CFU) counting, protease activity assay, and pyocyanin and pyoverdine quantification were used to test the proliferation and production of virulence factors of the bacteria.

resultsCocultures of P. aeruginosa and S. aureus isolated from the same patient reduced HNEC TEER values, had an earlier onset of HNEC barrier disruption, and increased paracellular permeability compared to monocultures of P. aeruginosa. P. aeruginosa proliferation was enhanced, and protease activity increased significantly. The production of pyoverdine increased significantly in the same patient cocultures, while the pyocyanin levels remained unchanged.

conclusionsThese results indicate a role of within-host evolution in shaping P. aeruginosa-mediated virulence in the context of polymicrobial biofilms. This supports the need to develop strategies directed at disrupting interspecies synergies that culminate in the formation of polymicrobial biofilms associated with CRS for the purpose of improving disease management and therapeutic efficacy.

Indexed as

Epithelial CellsNasal MucosaPseudomonas aeruginosaPseudomonas InfectionsSinusitisStaphylococcal InfectionsStaphylococcus aureusBiofilmsCells, CulturedChronic DiseaseCoculture TechniquesHumansVirulenceVirulence FactorsVirulence Factorsbiofilmschronic rhinosinusitisepithelial barrier integritypolymicrobial infectionsPseudomonas aeruginosaStaphylococcus aureus

Identifiers

PMID41025540
PMCPMC12761362

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.