Astrocytic modulation of population encoding in mouse visual cortex via GABA transporter 3 revealed by multiplexed CRISPR/Cas9 gene editing.
Jiho Park, Grayson O Sipe, Xin Tang, Prachi Ojha, Giselle Fernandes, Yi Ning Leow, Caroline Zhang, Yuma Osako, Arundhati Natesan, Gabrielle T Drummond and 2 more
Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1citing papers in PubMed
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1 · What the graph read from it
What it found
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2 · The registry
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Grayson O Sipe *Department of Biology, Eberly College of Science and Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, United States.
Xin TangF.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, United States.
Prachi OjhaPicower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, United States.
Neuron-astrocyte mechanisms of norepinephrine in goal-directed learningR01MH133066 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI MRIGANKA SUR · 2023 to 2026
$2.6M
Astrocyte-Neuron Circuits Underlying Cortical Mechanisms of Learned BehaviorR01NS130361 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI MRIGANKA SUR · 2022 to 2026
$2.1M
Spatiotemporal dynamics of locus coeruleus circuits during learned behaviorR01MH126351 · NIMH · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI SUR, MRIGANKA · 2021 to 2025
$2.1M
Novel tools for spatiotemporal modulation of astrocytes in neuronal circuitsRF1DA049005 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI SUR, MRIGANKA · 2019 to 2019
$1.5M
Role of glial glutamate transporters in V1 plasticity and developmentF32EY022264 · NEI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI PETRAVICZ, JEREMY C · 2012 to 2014
$163k
JPB Foundation Picower Postdoctoral FellowshipNEI NIH HHS F32 EY022264NIDA NIH HHS RF1 DA049005NIH HHS F32EY022264NIH HHS R01DA049005NIH HHS R01MH126351NIH HHS R01MH133066NIH HHS R01NS130361NIMH NIH HHS R01 MH126351NIMH NIH HHS R01 MH133066NINDS NIH HHS R01 NS130361Simons Foundation Autism Research Initiative Bridge to Independence awardUnited States Department of Defense Multidisciplinary University Research Initiative W911NF2110328
6 · The paper itself
Abstract
Astrocytes, which are increasingly recognized as pivotal constituents of brain circuits governing a wide range of functions, express GABA transporter 3 (Gat3), an astrocyte-specific GABA transporter responsible for maintenance of extra-synaptic GABA levels. Here, we examined the functional role of Gat3 in astrocyte-mediated modulation of neuronal activity and information encoding. First, we developed a multiplexed CRISPR construct applicable for effective genetic ablation of Gat3 in the visual cortex of adult mice. Using
Indexed as
AstrocytesCRISPR-Cas SystemsGABA Plasma Membrane Transport ProteinsGene EditingVisual CortexAnimalsMiceMice, KnockoutNeuronsGABA Plasma Membrane Transport Proteinsastrocytesgenetic engineeringinformation processinginhibitory transmissionmouseneurosciencepopulation dynamicsvisual cortex
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Astrocytic modulation of population encoding in mouse visual cortex via GABA transporter 3 revealed by multiplexed CRISPR/Cas9 gene editing. · full record | OpenQuestion