Evidence map›Paper›PMID 41024616›Full record

ArticleBrain and behavior2025

Enhanced Fear Memory in Adult Male C57BL/6 Mice Following Low-Dose Isoflurane Exposure During Juvenile Development.

Jing Sun, Yong Tang, Jinlong Chang, Jianbang Lin, Li Luo, Yuantao Li, Guanxiong Wu, Yuting Hu, Zhao Zheng, Ye Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain and behavior, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jing SunDepartment of Anesthesiology, Shenzhen Futian District Maternal and Child Health Hospital, Shenzhen, China.ORCID https://orcid.org/0000-0002-6459-1255
Yong TangBrain Cognition and Brain Disease Institute, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Jinlong ChangDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Dali University, Dali, China.
Jianbang LinShenzhen Technological Research Center for Primate Translational Medicine, Shenzhen Key Laboratory for Molecular Biology of Neural Development, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Li LuoDepartment of Anesthesiology, Shenzhen Futian District Maternal and Child Health Hospital, Shenzhen, China.
Yuantao LiShenzhen Maternity and Child Healthcare Hospital, Women and Children's Medical Center, Southern Medical University, Shenzhen, China.
Guanxiong WuShenzhen Maternity and Child Healthcare Hospital, Women and Children's Medical Center, Southern Medical University, Shenzhen, China.
Yuting HuShenzhen Maternity and Child Healthcare Hospital, Women and Children's Medical Center, Southern Medical University, Shenzhen, China.
Zhao ZhengShenzhen Maternity and Child Healthcare Hospital, Women and Children's Medical Center, Southern Medical University, Shenzhen, China.
Ye ZhangDepartment of Traditional Chinese Medicine, Shenzhen Futian District Maternal and Child Health Hospital, Shenzhen, China.

Funding

Shenzhen Science and Technology Innovation Commission JCYJ20230807120302004Wellcome Trust 202575Yunnan Fundamental Research Project 202501CF070194
6 · The paper itself

Abstract

objectiveTo elucidate the neural mechanisms through which neonatal exposure to low-dose anesthetic isoflurane (ISO) enhances adult fear memory, specifically examining neural oscillations and activation patterns in the mediodorsal thalamus (MDL), dentate gyrus (DG), anterior cingulate cortex (ACC), and medial entorhinal cortex (MEC).

methodsMale C57BL/6 mice received 0.75% ISO or saline injections at postnatal day 7 (P7). At 6 months, contextual fear-memory testing was conducted (Saline + Air: n = 14; Saline + ISO: n = 13). Neural correlates were assessed via in vivo electrophysiology during behavioral tasks and c-Fos immunohistochemistry in the MDL, DG, ACC, and MEC.

resultsISO-exposed mice exhibited significantly enhanced fear memory, with >75% displaying freezing as the dominant conditional response (vs. controls). This was accompanied by increased c-Fos+ cell density in the MDL and altered slow gamma oscillation power in fear-processing circuits. Non-freezing responses (e.g., jumping/darting) were reduced in the Saline + ISO group.

interpretationEarly low-dose ISO intervention in neonatal mice enhances adult fear memory, potentially through MDL activation and slow gamma oscillation modulation. These findings indicate that low-dose ISO may improve cognitive function by optimizing fear memory consolidation, contrasting with known detrimental effects of high-dose anesthetics on neurodevelopment.

Indexed as

Anesthetics, InhalationFearIsofluraneMemoryAnimalsAnimals, NewbornBrainEntorhinal CortexMaleMiceMice, Inbred C57BLProto-Oncogene Proteins c-fosAnesthetics, InhalationIsofluraneProto-Oncogene Proteins c-fosfearisofluranesafety assessmentsedationslow gamma oscillationsspatial cognition

Identifiers

PMID41024616
PMCPMC12480919

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.