Evidence map›Paper›PMID 41024519›Full record

ArticleParasitology2025

Development of chimeric multivalent proteins for serological diagnosis of African animal trypanosomosis.

Robert Eustache Hounyèmè, Loïc Rivière, Antoine Abel Missihoun, Veerle Lejon, Akpole Koffi Kouakou, Christina Calmels, Zakaria Bengaly, Casmir Akpovi, Souaibou Farougou, Sophie Thévenon and 2 more

Abstract read
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Article in Parasitology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Robert Eustache HounyèmèUnité de recherche et laboratoire de trypanosomiase et leishmaniose, Institut Pierre Richet, Bouaké, Côte d'Ivoire.ORCID https://orcid.org/0000-0003-0097-6129
Loïc RivièreMicrobiologie Fondamentale et Pathogénicité, Université de Bordeaux, CNRS UMR 5234, Bordeaux, France.
Antoine Abel MissihounDépartement de Génétique et des Biotechnologies, Faculté des Sciences et Techniques (FAST), Université d'Abomey-Calavi, Cotonou, Bénin.
Veerle LejonINTERTRYP, Université de Montpellier, CIRAD, IRD, Montpellier, France.
Akpole Koffi KouakouUnité de recherche et laboratoire de trypanosomiase et leishmaniose, Institut Pierre Richet, Bouaké, Côte d'Ivoire.
Christina CalmelsMicrobiologie Fondamentale et Pathogénicité, Université de Bordeaux, CNRS UMR 5234, Bordeaux, France.
Zakaria BengalyUnité de recherche sur les maladies à vecteurs et biodiversité, Centre International of Recherche-Développement sur l'Elevage en zone Subhumide (CIRDES), Bobo-Dioulasso, Burkina Faso.
Casmir AkpoviDépartement de Génétique et des Biotechnologies, Faculté des Sciences et Techniques (FAST), Université d'Abomey-Calavi, Cotonou, Bénin.
Souaibou FarougouDépartement de Génétique et des Biotechnologies, Faculté des Sciences et Techniques (FAST), Université d'Abomey-Calavi, Cotonou, Bénin.
Sophie ThévenonINTERTRYP, Université de Montpellier, CIRAD, IRD, Montpellier, France.
Dramane KabaUnité de recherche et laboratoire de trypanosomiase et leishmaniose, Institut Pierre Richet, Bouaké, Côte d'Ivoire.
Alain BoulangéUnité de recherche et laboratoire de trypanosomiase et leishmaniose, Institut Pierre Richet, Bouaké, Côte d'Ivoire.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The control of African animal trypanosomosis (AAT) relies on accurate diagnostic tools. Serological diagnosis using ELISA is well-suited for surveillance due to its high-throughput capacity, low cost, and adaptability to rapid formats. However, the WOAH-recommended antibody ELISA for AAT, based on trypanosome lysates purified from rodent blood, can lack specificity and presents standardization challenges as well as ethical concerns. Recombinant proteins offer a solution to standardization, often improving specificity, though potentially at the expense of sensitivity. Combining multiple recombinant proteins can enhance sensitivity while maintaining specificity. Therefore, this study developed chimeric proteins for serological diagnosis of AAT, composed of highly immunoreactive regions from multiple known antigens using genetic engineering. Following an inventory of immunodominant antigens, we selected candidates and, using bioinformatics, designed five chimeric constructs

Indexed as

Antigens, ProtozoanCattle DiseasesTrypanosomaTrypanosomiasis, AfricanTrypanosomiasis, BovineAnimalsAntibodies, ProtozoanCattleEnzyme-Linked Immunosorbent AssayRecombinant Fusion ProteinsRecombinant ProteinsSensitivity and SpecificitySerologic TestsAntibodies, ProtozoanAntigens, ProtozoanRecombinant Fusion ProteinsRecombinant ProteinsAfrican animal trypanosomosischimeric multivalent antigendiagnosisgenetic engineeringTrypanosoma bruceiTrypanosoma congolenseTrypanosoma vivax

Identifiers

PMID41024519
PMCPMC12921242

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.