Evidence map›Paper›PMID 41024280›Full record

ArticleStem cell research & therapy2025

A GelMA/polydopamine hydrogel with PTH and osteogenically stimulated alveolar mucosa-derived stem cells promotes bone regeneration in MRONJ-affected wounds.

Che-Chang Tu, Ming-Hsu Chen, Guan-Yu Lan, Yu-Ting Lin, Yu-Tse Lin, Wei-Chiu Tai, Jiashing Yu, Po-Chun Chang

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Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Che-Chang Tu *Department of Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan.
Ming-Hsu Chen *Department of Otolaryngology, Cathay General Hospital, Taipei, Taiwan.
Guan-Yu LanDepartment of Chemical Engineering, College of Engineering, National Taiwan University, Taipei, Taiwan.
Yu-Ting LinDepartment of Chemical Engineering, College of Engineering, National Taiwan University, Taipei, Taiwan.
Yu-Tse LinDepartment of Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan.
Wei-Chiu TaiGraduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Jiashing YuDepartment of Chemical Engineering, College of Engineering, National Taiwan University, Taipei, Taiwan.
Po-Chun ChangDepartment of Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan. changpc@ntu.edu.tw.

Funding

National Science and Technology Council 110-2314-B-002-109-MY3National Science and Technology Council 113-2314-B-002-039National Taiwan University Hospital 112-CGN0004
6 · The paper itself

Abstract

backgroundMedication-related osteonecrosis of the jaw (MRONJ) is a serious complication in patients taking bisphosphonates. This study aimed at developing a mesenchymal stem cell-based strategy to reduce the incidence of MRONJ and recover regeneration capability of MRONJ-affected wounds by using a gelatin methacryloyl/polydopamine hydrogel (GelMA/PD) to adhere alveolar mucosa-derived stem cells (AMCs) on the bone surface, with the osteogenically stimulated AMCs modulated by microRNA (miR) transfection, and the osteoanabolic environment activated by parathyroid hormone (PTH).

methodsGelMA/PD was synthesized by photo-crosslinking, and the incorporation of PD onto GelMA as well as mechanical properties were assessed. Rat AMCs were isolated, and the stemness was characterized. AMCs were osteogenically stimulated by miR transfection. Maxillary osteotomy was created in rats administrated with zoledronic acid and dexamethasone to simulate MRONJ-affected wounds, and osteotomy in rats without ZA served as healthy controls. Wounds were unfilled or filled with GelMA/PD alone, GelMA/PD with AMCs (GA), GelMA/PD with OAMCs (GO), or GelMA/PD with OAMCs and PTH (PO), and were assessed by gross observation, micro-CT imaging, histology, and immunohistochemistry for osteoblast-osteoclast coupling.

resultsGelMA/PD exhibited modestly decreased compressive strength and superior adhesion strength compared with GelMA. AMCs were double positive for CD73 and CD90, showed trilineage differentiation capability, and were osteogenically stimulated by miR-218 transfection. Among MRONJ-affected wounds, soft tissue coverage was accelerated, with reduced sequestra and significantly greater bone volume in PO group (38.46 ± 10.02%) relative to unfilled group (21.81 ± 6.18%), and osteoblast-osteoclast coupling was evident in GO and PO groups. Soft tissue recovery, inflammation reduction, and matrix deposition on defect surfaces were more prominent in PO group.

conclusionGelMA/PD loaded with PTH and microRNA-218-transfected AMCs could facilitate mucosal healing, recover the osteoblast-osteoclast coupling, and repair MRONJ-affected wounds, and might be a feasible strategy for managing MRONJ.

Indexed as

Bone RegenerationHydrogelsIndolesOsteogenesisParathyroid HormonePolymersAnimalsGelatinMaleMesenchymal Stem CellsMicroRNAsRatsRats, Sprague-DawleyGelatinHydrogelsIndolesMicroRNAsParathyroid HormonepolydopaminePolymersBisphosphonate-associated osteonecrosis of the jawMesenchymal stem cellMicroRNAsParathyroid hormonePolydopamineWound healing

Identifiers

PMID41024280
PMCPMC12482642

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