Evidence map›Paper›PMID 41024186›Full record

ArticleMicrobial cell factories2025

Engineering the oleaginous yeast Yarrowia lipolytica for co-production of phenolic monoterpenes thymol and carvacrol.

Junkai Zhu, Yi Zhang, Haibo Jiang, Mingming Zheng, Yangmin Gong

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Article in Microbial cell factories, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Junkai Zhu *Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, 315211, Zhejiang, China.ORCID http://orcid.org/0009-0002-8692-1259
Yi Zhang *Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Haibo JiangKey Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, 315211, Zhejiang, China.ORCID http://orcid.org/0000-0002-3515-4398
Mingming ZhengKey Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. zhengmingming@caas.cn.ORCID http://orcid.org/0000-0002-4965-2451
Yangmin GongKey Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China. gongyangmin@caas.cn.ORCID http://orcid.org/0000-0002-4096-3640

Funding

Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences CAASASTIP-2013-OCRIHubei Province Technology Innovation Programme 2024BBB040
6 · The paper itself

Abstract

backgroundThymol and carvacrol are natural phenolic compounds with various biological activities. They are widely used in the spice and medicine industries. Production of thymol and carvacrol using microbial cell factories is considered a viable alternative to extracting them from plants of the Thymus genus or the Lamiaceae family.

resultsIn this study, the complete synthetic pathways of thymol and carvacrol were constructed using the oleaginous yeast Yarrowia lipolytica Po1f as a chassis. The total titer of thymol and carvacrol was increased 18.44-fold by enhancing the mevalonate pathway during the modification process and reducing the metabolic flux from geranyl diphosphate (GPP) to farnesyl diphosphate (FPP) through the modification of ERG20. Next, by increasing the copy number of the TvCYP71D507 gene combination (TvCYP71D507 + TvTPS2 + TvSDR1) in the synthetic pathway, the total titer was increased by 1.75-fold. Finally, the engineered strain CT18, which was reintroduced the 3-isopropylmalate dehydrogenase (LEU2) gene, achieved a titer of thymol and carvacrol of 7.14 mg/L in shake flasks and 61.31 mg/L in a 5-L bioreactor.

conclusionThis study demonstrates the de novo synthesis of thymol and carvacrol in Y. lipolytica for the first time and provides a valuable reference for constructing microbial cell factories for phenolic monoterpenes biosynthesis.

Indexed as

Metabolic EngineeringMonoterpenesThymolYarrowiaCymenescarvacrolCymenesMonoterpenesThymolBioproductionCarvacrolSynthetic biologyThymolYarrowia lipolytica

Identifiers

PMID41024186
PMCPMC12482179

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