Evidence map›Paper›PMID 41023860›Full record

ArticleBMC genomics2025

Photoperiodic responsiveness in the DNA methylation and gene expression in the hypothalamus of ovariectomized and estradiol-treated ewes.

Xiaoyun He, Ran Di, Xiangyu Wang, Xiaofei Guo, Xiaosheng Zhang, Jinlong Zhang, Qiuyue Liu, Mingxing Chu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaoyun HeState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Ran DiState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Xiangyu WangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Xiaofei GuoTianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
Xiaosheng ZhangTianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
Jinlong ZhangTianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin Engineering Research Center of Animal Healthy Farming, Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, 300381, China.
Qiuyue LiuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. qyliu@genetics.ac.cn.
Mingxing ChuState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. mxchu@263.net.

Funding

the National Natural Science Foundation of China 32102508
6 · The paper itself

Abstract

backgroundSheep are short-day breeder, and their reproductvie activity can be activated by short photoperiods, but the regulatory mechanism remains unclear. In this study, hypothalamic tissues were collected from ovariectomized and estradiol-treated (OVX + E

resultsThe DNA methylation profiles of the hypothalamus were examined, and whole-genome single-base resolution methylome maps of the sheep hypothalamus were generated across seven time points under various photoperiod treatments. The results revealed that photoperiod-induced changes in genome-wide DNA methylation constitute a dynamic and reversible regulatory process, with a potential transition point occurring approximately 15 days after the switch from SP to LP. Following integrated analysis of differentially methylated regions-related genes (DMRGs) and differentially expressed genes (DEGs), it showed that the DNA methylation levels before the transcription start site (TSS) of the overlapping gene were different, and a negative correlation between gene expression levels and mCG levels in gene promoters was found (P < 0.01). Functional analysis of the overlapping genes revealed that pathways involved in synapse development, thyroid hormone signaling, and circadian rhythm were regulated by photoperiod-induced DNA methylation, thereby influencing hypothalamic function in photoperiod-dependent seasonal reproduction.

conclusionThis study generated the whole-genome single-base resolution DNA methylome maps of sheep hypothalamus under different photoperiods, and elucidated the regulatory relationship between DNA methylation and photoperiod-dependent seasonal reproduction in sheep hypothalamus tissues. Our findings provided a valuable resource for further research on the underlying mechanisms of the hypothalamic-regulated reproductive seasonality in sheep.

Indexed as

DNA MethylationEstradiolGene Expression RegulationHypothalamusPhotoperiodAnimalsFemaleGene Expression ProfilingOvariectomySheepEstradiolDNA methylationHypothalamusPhotoperiodSeasonal reproductionSheep

Identifiers

PMID41023860
PMCPMC12482692

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.