Evidence map›Paper›PMID 41023668›Full record

ArticleJournal of nanobiotechnology2025

Dimensional control of DNA nanostructures enhances cellular uptake and guides tissue-regenerative responses.

Xinyue Tang, Tingting Zhai, Tiancheng Li, Yu Jin, Dantong Lei, Cheng Zhu, Luyao Qu, Yingfu Li, Yudong Wang, Hongzhou Gu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. DNA Nanostructures for siRNA Delivery.Bioconjugate chemistry · 2026
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xinyue Tang *Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China.
Tingting Zhai *State Key Laboratory of Synergistic Chem-Bio Synthesis, Department of Chemical Biology, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China. zhaitingting@sjtu.edu.cn.
Tiancheng LiDepartment of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China.
Yu JinDepartment of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China.
Dantong LeiState Key Laboratory of Synergistic Chem-Bio Synthesis, Department of Chemical Biology, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
Cheng ZhuDepartment of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China.
Luyao QuDepartment of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China.
Yingfu LiDepartment of Biochemistry and Biomedical Sciences and Michael G. DeGroote Institute of Infectious Disease Research, McMaster University, Ontario, L8S 4K1, Canada.
Yudong WangDepartment of Gynecologic Oncology, School of Medicine, The International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai, 200030, China.
Hongzhou GuState Key Laboratory of Synergistic Chem-Bio Synthesis, Department of Chemical Biology, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China. hongzhou.gu@sjtu.edu.cn.
Bing FangDepartment of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China. fangbing@sjtu.edu.cn.

Funding

National Key Research and Development Program of China 2020YFA0908901National Natural Science Foundation of China 32171348National Natural Science Foundation of China 82121002the Program of Shanghai Academic Research Leader 22XD1421500
6 · The paper itself

Abstract

Precise regulation of cellular functions is fundamental for advancing tissue regeneration and drug delivery systems. Structural DNA nanotechnology enables the design of well-defined nanostructures, emerging as a promising platform in these biomedical applications. However, a clear understanding of how the dimensional properties of DNA nanostructures affect cellular uptake and biological responses remains limited. In this study, we constructed three distinct DNA nanostructures: a one-dimensional six-helix bundle (6HB), a two-dimensional three-point star, and a three-dimensional tetrahedron. We systematically evaluated their endocytic efficiency in five representative cell types: endothelial cells, dermal fibroblasts, myoblasts, chondrocytes, and osteoblasts. Among them, the 6HB exhibited the highest cellular uptake, with minimal variability across cell types in both 2D petri dish cultures and 3D multicellular spheroid invasion models. Moreover, DNA nanostructures were found to enhance cell proliferation in fibroblasts and chondrocytes, support chondrocyte phenotype maintenance, and, in the case of the 6HB, promote myoblast differentiation. These findings provide new insights into structure-function relationships in DNA nanomaterials and offer guidance for optimizing DNA-based platforms for drug delivery and regenerative medicine.

Indexed as

DNANanostructuresAnimalsCell DifferentiationCell ProliferationChondrocytesDrug Delivery SystemsEndocytosisEndothelial CellsFibroblastsHumansMiceMyoblastsOsteoblastsRegenerative MedicineDNACellular uptakeDimensional controlDNA nanotechnologySix-helix bundleTissue regeneration

Identifiers

PMID41023668
PMCPMC12482324

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.