Evidence map›Paper›PMID 41022734›Full record

ArticleNature communications2025

In vivo CRISPR screening in head and neck cancer reveals Uchl5 as an immunotherapy target.

Cong Fu, Robert Saddawi-Konefka, Jordan M Chinai, Sarah Y Kim, Ashwin V Kammula, Jonathan J Perera, Aiping Jiang, Payal Tiwari, Erin N Kistler, Shiqi Tang and 19 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Cong FuDepartment of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Massachusetts, USA.
Robert Saddawi-KonefkaDepartment of Otolaryngology-Head and Neck Surgery, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0002-9936-1695
Jordan M ChinaiDepartment of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Massachusetts, USA.
Sarah Y KimBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Ashwin V KammulaBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-5284-721X
Jonathan J PereraBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-3224-6075
Aiping JiangBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Payal TiwariDepartment of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Massachusetts, USA.
Erin N KistlerBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Shiqi TangDepartment of Otolaryngology-Head and Neck Surgery, University of California San Diego, La Jolla, CA, USA.
Sarah M LunaMoores Cancer Center, University of California, San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0009-0002-7211-5539
Kayla J ColvinBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Juan DubrotBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-8093-2208
Seth AndersonBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-2419-0585
Rachel A FettermanBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Cun Lan ChuongBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Sarah K Lane-RetickerBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Collins K CheruiyotBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Audrey J MuscatoBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-2009-6313
Zahra AlipourPerelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Douglas R AdkinsDivision of Medical Oncology, Washington University School of Medicine, Robert Ebert and Greg Stubblefield Head and Neck Tumor Center, Alvin J. Siteman Cancer Center, and Barnes Jewish Hospital, St. Louis, MO, USA.
Gabriel K GriffinBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-4042-6757
Bradley E BernsteinBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Ann Marie EgloffDepartment of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Massachusetts, USA.
Kathleen B YatesBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-7383-5573
Rebecca D ChernockDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
J Silvio GutkindMoores Cancer Center, University of California, San Diego, La Jolla, CA, USA.
Ravindra UppaluriDepartment of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Massachusetts, USA. ravindra_uppaluri@dfci.harvard.edu.ORCID http://orcid.org/0000-0001-5988-6828
Robert T MangusoBroad Institute of MIT and Harvard, Cambridge, MA, USA. rmanguso@broadinstitute.org.ORCID http://orcid.org/0000-0003-1336-413X

Funding

Defining mechanisms of immunotherapy resistance in head and neck squamous cell carcinomasU01DE029188 · NIDCR · DANA-FARBER CANCER INST · PI BARBIE, DAVID A, HADDAD, ROBERT I. · 2019 to 2019
$4.3M
U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research (NIDCR) U01DE029188
6 · The paper itself

Abstract

Recurrent/metastatic head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with a significant unmet need for enhancing immunotherapy response given current modest efficacy. Here, we perform an in vivo CRISPR screen in an HNSCC mouse model to identify immune evasion genes. We identify several regulators of immune checkpoint blockade (ICB) response, including the ubiquitin C-terminal hydrolase 5 (UCHL5). Loss of Uchl5 in tumors increases CD8

Indexed as

Head and Neck NeoplasmsImmunotherapySquamous Cell Carcinoma of Head and NeckUbiquitin ThiolesteraseAnimalsCD8-Positive T-LymphocytesCell Line, TumorClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsEpithelial-Mesenchymal TransitionExtracellular MatrixFemaleGene Expression Regulation, NeoplasticHumansImmune Checkpoint InhibitorsMiceImmune Checkpoint InhibitorsUbiquitin Thiolesterase

Identifiers

PMID41022734
PMCPMC12480505

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.