ArticleVeterinary medicine and science2025
Subtype Identification of Blastocystis sp. Among Humans and Domestic Animals in the West of Iran.
Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- No Trace of Blastocystis Parasite in Bats Caught in the Western and Southwestern Regions of Iran.Veterinary medicine and science · 2026Article
- Molecular Prevalence and Genetic Diversity of Blastocystis sp. in Slaughtered Ruminants in Qazvin Province, Iran: A Zoonotic Concern.Veterinary medicine and science · 2026Article
- Subtype Identification of Blastocystis sp. Among Humans and Domestic Animals in the West of Iran.Veterinary medicine and science · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
backgroundBlastocystis is a genetically diverse intestinal protozoan found in a wide range of human and animal hosts. The present study was conducted to identify the subtypes of Blastocystis in both humans and animals in Kermanshah province, Iran.
methodsA total of 820 stool samples from humans, cattle, sheep, and pigeons were examined using the formalin-ethyl acetate concentration method. All isolates were cultured in clotted fetal bovine serum medium. DNA extraction, PCR amplification and sequencing were performed targeting an identical fragment of the small subunit ribosomal RNA (SSU rRNA) gene. Phylogenetic tree and haplotype network were conducted by MEGA6 and PopART software. Data analysis was performed by Chi-square and Fisher's exact tests, while a p value of < 0.05 was considered as statistically significant.
resultsTotally, Blastocyctis was found in 9.1% and 4.5% of human samples using microscopy and growing in culture medium, respectively. A significant association was observed between Blastocystis infection and both age and gastrointestinal symptoms. Two subtypes of ST2 and ST5 were identified in humans and livestock (cattle and sheep), respectively. The results of haplotype analysis showed clear classification of two groups and six haplotype (Group I and II) in the isolates.
conclusionThis study highlights the zoonotic potential of Blastocystis species in the studied region. The subtype distribution may inform preventive strategies and help elucidate transmission routes. Additionally, further research is needed to fully understand the pathogenic potential of this parasite.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.