Evidence map›Paper›PMID 41020662›Full record

ArticleVeterinary medicine and science2025

Detection of Genetic Polymorphism in Growth Hormone Receptor Gene and Its Influence on Growth Traits in Kari and Balkhi Sheep.

Hanan Al-Khalaifah, Muniza Kiran, Sher Hayat Khan, Ihtesham Ulhaq, Muhammad Ibrahim, Sohail Ahmad, Haris Bilal, Zeng Shenming, Shabana Naz, Rifat Ullah Khan and 1 more

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Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hanan Al-KhalaifahEnvironment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Safat, Kuwait.
Muniza KiranInstitute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Sher Hayat KhanInstitute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Ihtesham UlhaqInstitute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Muhammad IbrahimInstitute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Sohail AhmadInstitute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Haris BilalInstitute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Zeng ShenmingState Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Shabana NazDepartment of Zoology, Government College University Faisalabad, Faisalabad, Pakistan.
Rifat Ullah KhanPhysiology Lab, College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Ibrahim A AlhidaryDepartment of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Variations in the growth hormone receptor (GHR) gene influence growth traits, which are important for economic selection in sheep breeding. This study aimed to investigate GHR gene sequence variations and their association with growth traits in two sheep breeds (Kari and Balkhi). A total of 150 animals per breed were included, and blood samples were collected. DNA was extracted using the NucleoSpin Blood Genomic DNA Purification Kit, and the gene was amplified through PCR with pre-designed primers. PCR products were sequenced using next-generation sequencing (NGS). Sequence analysis identified 620 variants, with 612 located in the intronic region. Five variants were found in the upstream region, and two synonymous variants were detected, along with one in the 3' UTR region. Correlation analysis showed that four SNPs (33,665,691 C>T, 33,668,886 T>A, 33,665,855 C>T and 33,667,058 C>T) in high-growth rate groups were positively correlated with weight gain, while four SNPs (33,662,328 G>A, 33,729,972 C>T, 33,753,532 A>G and 33,760,254 G>T) in low-growth rate groups were negatively correlated. These SNPs could serve as markers for marker-assisted selection to improve growth traits in local sheep breeds.

Indexed as

Polymorphism, Single NucleotideReceptors, SomatotropinSheep, DomesticAnimalsFemaleMaleSheepReceptors, SomatotropinBalkhi sheepgrowth hormone receptorKari sheepNGSSNP

Identifiers

PMID41020662
PMCPMC12477801

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.