Evidence map›Paper›PMID 41020595›Full record

ArticlemSphere2025

A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi.

Alison C E Wirshing, Cristina Colino-Palomino, Analeigha V Colarusso, Mario Pinar, Daniel J Lew

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Allocation of resources among multiple daughter cells.bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Alison C E WirshingDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.ORCID 0000-0002-2362-0531
Cristina Colino-PalominoDepartment of Molecular and Cellular Biosciences, CSIC Centro de Investigaciones Biológicas Margarita Salas, Madrid, Spain.
Analeigha V ColarussoDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Mario PinarDepartment of Molecular and Cellular Biosciences, CSIC Centro de Investigaciones Biológicas Margarita Salas, Madrid, Spain.
Daniel J LewDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.ORCID 0000-0001-7482-3585

Funding

Studies on cell polarity, chemotropism, and cell cycle controlR35GM122488 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI DANIEL J LEW · 2017 to 2026
$6.9M
Agencia Estatal de Investigación PID2021-124278OB-I00Agencia Estatal de Investigación PRE22-101869Agencia Estatal de Investigación TED2021-129607B-I00NIGMS NIH HHS R35 GM122488NIGMS NIH HHS R35GM122488
6 · The paper itself

Abstract

Actin is highly conserved across eukaryotes. This versatile protein builds cytoskeletal networks central to diverse cellular processes, including cell division and cell motility. The most potent and broadly used reagents to detect polymerized actin distribution in fixed cells are fluorescently conjugated derivatives of the basidiomycete-derived toxin, phalloidin. However, despite its conservation, actin in many ascomycete fungi fails to bind phalloidin. Here, we trace the failure to bind phalloidin to a single amino acid change in a phalloidin-binding residue in actin. Reverting this change in the fungi

Indexed as

ActinsAspergillus nidulansFungiPhalloidineStaining and LabelingActinsPhalloidineactinfilamentous fungiphalloidin

Identifiers

PMID41020595
PMCPMC12570480

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.