ArticleNucleic acids research2025
Eps2Fold: a rapid method to characterize G-quadruplex DNA structures using single absorbance spectra.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- NA-CDQuant: Quantitative spectral analysis of nucleic acid structure by circular dichroism spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Dibenzoacridinium derivatives: a new class of G-quadruplex ligands with anti-HIV-1 properties.RSC medicinal chemistry · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
G-rich oligonucleotides can fold into non-canonical secondary structures called G-quadruplexes (G4s), consisting of stacked G-tetrads, i.e. planar arrays of four guanines connected by eight hydrogen bonds and coordinating cations such as K+ and Na+. G4s are remarkably polymorphic structures, which enables them to participate in various biological processes and find applications in nanotechnology. The development of simple and rapid assays to better understand how and when G4s form remains very important. Here, we present Eps2Fold (Epsilon-to-Fold), a method for detection and characterization of G4 conformations by simply measuring a single UV-absorbance spectrum. Eps2Fold is conceptually similar to isothermal differential spectra or thermal differential spectra but is generated by the subtraction of an experimentally recorded UV absorbance spectrum of the G4 in its folded state and a calculated UV absorbance spectrum that mimics the G4 in its unfolded state. Through comprehensive biophysical and principal component analysis (PCA) analysis of thirty G4-forming oligonucleotides, we demonstrate that Eps2Fold provides spectral signatures with unexpectedly high structural resolution remarkably similar to that obtained with circular dichroism spectra. Both techniques allow precise topology assignment based on the number and composition of stacked syn or anti guanines glycosidic bond angle stacks. The spectral differences between conformers are subtle and best analyzed using multivariate statistical approaches such as PCA rather than visual inspection alone. We provide an open-source GUI program to facilitate such analysis. Users simply upload the UV absorbance spectrum of the G4 on the web application (https://github.com/EricLarG4/Eps2Fold); structural analysis will run automatically. Eps2Fold represents a rapid and inexpensive structural approach enabling precise characterization of G4 structures from a single UV absorbance spectrum.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.