ArticleFrontiers in plant science2025
Optimized LC-MS method for simultaneous polyamine profiling and ADC/ODC activity quantification and evidence that ADCs are indispensable for flower development in tomato.
Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Polyamines (PAs) are essential for plant development and stress responses, requiring tight homeostatic regulation. As many PA enzymes are regulated post-transcriptionally, transcript-based methods cannot accurately predict protein abundance. This limitation highlights the need for alternative approaches to study PA homeostasis. Methods: We optimized a liquid chromatography-mass spectrometry (LC-MS)-based method to simultaneously quantify the activities of two key PA-synthesizing enzymes-arginine decarboxylase (ADC) and ornithine decarboxylase (ODC)-from plant tissues using stable isotope substrates. Substrate concentrations were optimized to increase assay sensitivity, and the method was adapted for Results: In tomato leaf tissue, assay sensitivity improved more than tenfold. In Discussion: Together, the optimized LC-MS method, the ability to functionally analyze recombinant ADC/ODC proteins
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