ArticleBiology methods & protocols2025
A robust protocol for proteomic profiling of secreted proteins in conditioned culture medium.
Article in Biology methods & protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Automated Microvolume Secretome Proteomics Enables Sensitive and Deep Profiling and Noninvasive Single-Embryo Analysis.Journal of proteome research · 2026Article
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4 authors.
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Abstract
Reliable secretome analysis is crucial for understanding cellular communication and developing therapeutic strategies. However, conventional protein quantification methods, such as the bicinchoninic acid (BCA) assay, can overestimate protein concentrations in concentrated culture media, leading to inconsistent protein loading and compromised quantitative accuracy in mass spectrometry-based proteomics. To address this methodological challenge, we developed an improved sample preparation method for secretome analysis. Our approach introduces a concentration rate-based normalization method that adjusts sample volumes according to the ultrafiltration concentration ratio, ensuring more consistent protein loading across samples. This method enabled reliable identification of 3468 secreted proteins with high reproducibility (
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