RNA 2'-OH modification with stable reagents enabled by nucleophilic catalysis.

Eric T Kool, Sumon Pratihar, Pavitra S Thacker, Dipanwita Banerjee, Moon Jung Kim, Ryuta Shioi

Abstract read

In one paragraph

Article in RSC advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Eric T KoolDepartment of Chemistry, Stanford University Stanford California USA kool@stanford.edu.ORCID https://orcid.org/0000-0002-7310-2935

Sumon PratiharDepartment of Chemistry, Stanford University Stanford California USA kool@stanford.edu.

Pavitra S ThackerDepartment of Chemistry, Stanford University Stanford California USA kool@stanford.edu.

Dipanwita BanerjeeDepartment of Chemistry, Stanford University Stanford California USA kool@stanford.edu.

Moon Jung KimDepartment of Chemistry, Stanford University Stanford California USA kool@stanford.edu.

Ryuta ShioiDepartment of Chemistry, Stanford University Stanford California USA kool@stanford.edu.

Funding

Transcriptome Analysis with RNA-Reactive ProbesR35GM145357 · NIGMS · STANFORD UNIVERSITY · PI ERIC T. KOOL · 2022 to 2026

$3.0M

NIGMS NIH HHS R35 GM145357

6 · The paper itself

Abstract

RNA modification at 2'-OH has typically required highly reactive acylating species that exhibit short half-lives in water, challenging purification, and limiting shelf lives. Here, we investigate the use of more stable species as electrophilic reagents, employing nucleophilic catalysis to promote reactions. The results show that multiple previously unreported electrophiles can react in high stoichiometric yields with RNA under appropriate catalysis. Most notably, aryl esters can transfer acyl groups to RNA in one hour, but are stable for months even in pure water. The results expand the functional chemotypes of RNA-reactive species, and identify reagent classes with improved stability and selectivity.

Identifiers

PMID41018161

DOI10.1039/d5ra06314k

PMCPMC12466920

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.