Evidence map›Paper›PMID 41016985›Full record

ArticleMolecular biomedicine2025

5-Hydroxytryptamine promotes non-small cell lung cancer metastasis via the SNRPG/WT1/CDK14 Axis.

Jinzhe Sun, Chen-Guang Liu, Shiqian Chen, Huan Zhou, Xiangjun Liu, Fei-Ran Wang, Ya-Wen Luo, Dan Zang, Jun Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biomedicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jinzhe Sun *Department of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Chen-Guang Liu *Department of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Shiqian Chen *Department of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Huan Zhou *Department of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Xiangjun LiuDepartment of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Fei-Ran WangDepartment of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Ya-Wen LuoDepartment of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China.
Dan ZangDepartment of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China. danzang@dmu.edu.cn.
Jun ChenDepartment of Oncology, The Second Hospital of Dalian Medical University, Dalian, Liaoning, China. chenjun_dmu@126.com.

Funding

National Natural Science Foundation of China 82203056
6 · The paper itself

Abstract

Tumor metastasis severely compromises the prognosis of patients with non-small cell lung cancer (NSCLC), yet the underlying mechanisms remain unclear. The biogenic amine 5-hydroxytryptamine (5-HT), derived from tryptophan (Trp), has been reported to promote tumor growth, but its role in NSCLC metastasis has not been fully elucitated. In this study, fecal metabolomic profiling revealed significant enrichment of Trp metabolism and serotonergic synapse pathways in patients with NSCLC metastasis. Notably, the levels of 5-HT in peripheral blood showed a positive correlation with fecal Trp concentrations in NSCLC patients. Functional experiments demonstrated that 5-HT promoted NSCLC cell migration, invasion, epithelial-mesenchymal transition (EMT), and metastasis by inhibiting the expression of small nuclear ribonucleoprotein polypeptide G (SNRPG). RNA sequencing (RNA-seq) further identified cyclin-dependent kinase 14 (CDK14) as a critical downstream target of SNRPG. SNRPG downregulated CDK14 expression, thereby restraining malignant phenotypes in NSCLC cells. Furthermore, SNRPG negatively regulated the protein levels of Wilms tumor 1 (WT1) by directly binding to it. Loss of SNRPG led to WT1 accumulation, which in turn bound to the CDK14 promoter and activated transcription, ultimately driving NSCLC metastasis. These findings collectively suggest that the 5-HT/SNRPG/WT1/CDK14 axis plays a pivotal role in promoting NSCLC metastasis and highlight 5-HT and SNRPG as potential therapeutic targets for NSCLC metastasis.

Indexed as

Carcinoma, Non-Small-Cell LungCyclin-Dependent KinasesLung NeoplasmsSerotoninAnimalsCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHumansMaleMiceNeoplasm MetastasisSignal TransductionCyclin-Dependent KinasesSerotonin5-HydroxytryptamineCyclin-dependent kinase 14MetastasisNon-small cell lung cancerSmall nuclear ribonucleoprotein polypeptide G

Identifiers

PMID41016985
PMCPMC12477100

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.