ArticleJournal of microbiology and biotechnology2025
Development of a Multiplex TaqMan Real-Time RT-PCR Assay for the Rapid Differential Detection of Classic, MLB-Clade and VA-Clade Human Astroviruses.
Article in Journal of microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Astrovirus and norovirus infections and their association with diarrheal symptoms in immunocompromised children.Emerging microbes & infections · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The classical human astrovirus (HAstV) causes acute or severe gastroenteritis mainly among children and the elderly populations. The novel divergent HAstV-VA and HAstV-MLB clades related to encephalitis in immunocompromised patients, are genetically more similar to certain animal astroviruses, and have the potential for cross-species transmission. To identify all the three HAstV clades in a single reaction, we developed a quantitative reverse transcription PCR (RT-qPCR) method. Primers and probes were designed based on the conserved regions of the HAstV genomes that exhibited inter-clade divergence. This method was highly sensitive, as the detection limits were 95, 12.5 and 25 copies/μl for the classical HAstV, HAstV-MLB and HAstV-VA strains, respectively. Intra- and inter-assay variability revealed excellent reproducibility. Furthermore, this multiplex assay showed no cross-reactivity with other human pathogens. A total of 326 anal swabs from outpatients with AGE were examined: 5.83% were positive for the classical HAstV, 1.53% for the HAstV-MLB, and 0.31% for the HAstV-VA, while 0.31% for co-infections of HAstV-MLB/HAstV-VA. Conclusively, the developed multiplex RT-qPCR assay represents a tool with potential for laboratory and clinical diagnoses, epidemiological surveillance, prevention and control of the HAstV infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.