ArticleJournal of lipid research2025
Dietary phospholipid carriers of DHA do not increase brain DHA levels: a replication study.
Article in Journal of lipid research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DHA is primarily found in fish and seafood as triacylglycerols and phospholipids (PLs). Oral administration of PL DHA forms, sn-1 lysophosphatidylcholine-DHA (sn-1 LPC-DHA) and di-DHA phosphatidylcholine (di-DHA-PC), has been suggested to increase brain DHA levels by ∼100% (relative percent) and up to ∼200% (concentration) compared with controls. In contrast, triacylglycerol-DHA and nonesterified-DHA do not produce increases in brain DHA when provided in the diet. However, a subsequent study using a higher dose of sn-1 LPC-DHA did not confirm these findings and reported no significant increase in brain DHA. To address these inconsistencies, we aimed to replicate previous investigations of PL-DHA forms (LPC and PC) and their impact on brain DHA levels. Mice were randomly divided into one of four groups and received a daily gavage for 30 days of 80 μl of either corn oil alone (control) or corn oil containing 1 mg of DHA as nonesterified DHA, sn-1 LPC-DHA, or di-DHA-PC. DHA relative percent and concentrations were determined in brain regions (cortex, cerebellum, hippocampus, amygdala, striatum, and remainder of brain) and plasma using GC-flame ionization detection. Following treatment, no significant differences in DHA relative percent or concentration were observed between control and/or treatment groups in any brain region. Relative percent of plasma DHA was significantly elevated in all DHA-treated groups compared with the control group, confirming systemic absorption of the supplemented DHA. Our results demonstrate that dietary DHA provided as sn-1 LPC-DHA or di-DHA-PC does not increase brain DHA levels compared with nonesterified-DHA or the control group, failing to reproduce prior reports.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.