Evidence map›Paper›PMID 41014336›Full record

ArticleArchives of microbiology2025

Flow cytometric quantification of methanogens in anaerobic digestate.

Aatiya Shaikh, Srikanth Mutnuri

Abstract read
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In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Aatiya ShaikhApplied Environmental Biotechnology Laboratory, BITS Pilani, KK Birla Goa Campus, NH17B, Zuarinagar, Pilani, 403726, Goa, India.
Srikanth MutnuriApplied Environmental Biotechnology Laboratory, BITS Pilani, KK Birla Goa Campus, NH17B, Zuarinagar, Pilani, 403726, Goa, India. srikanth@goa.bits-pilani.ac.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Anaerobic digestion (AD) is an efficacious waste treatment technique that promotes organic stability and energy recovery. Methanogens are critical organisms that constitute the bottleneck in this process. The process relies on methanogenic archaea, which are crucial organisms that create a bottleneck. The quantitative assessment of these organisms can enhance process efficiency, identify issues prior to failure and result in community-focused process control systems. This paper introduces a flow cytometric method that is autofluorescence-based and used to rapidly and economically measure methanogenic archaea in a selection of microbial communities and raw substrates. The technique was applied on a sample of anaerobic digestate. The methanogenic archaea were quantified by measuring the specific fluorescence of their co-factor F

Indexed as

ArchaeaFlow CytometryMethaneAnaerobiosisBioreactorsMethanosarcinaMethane16S rRNA sequencingAnaerobic digestionAutofluorescenceF420Methanogenic archaeaSingle-cell analytics

Identifiers

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.