Evidence map›Paper›PMID 41013580›Full record

ArticleGenome medicine2025

Optimized high-throughput whole-genome sequencing workflow for surveillance of influenza A virus.

Matthias Licheri, Mike Mwanga, Manon F Licheri, Annika Graaf-Rau, Cora Sägesser, Pascal Bittel, Timm Harder, Franziska Suter-Riniker, Jenna N Kelly, Ronald Dijkman

Abstract read
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In one paragraph

Article in Genome medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Matthias Licheri *Institute for Infectious Diseases, University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland.
Mike Mwanga *Institute for Infectious Diseases, University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland.
Manon F LicheriInstitute for Infectious Diseases, University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland.
Annika Graaf-RauInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Cora SägesserInstitute for Infectious Diseases, University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland.
Pascal BittelInstitute for Infectious Diseases, University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland.
Timm HarderInstitute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
Franziska Suter-RinikerInstitute for Infectious Diseases, University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland.
Jenna N Kelly *Multidisciplinary Center for Infectious Diseases, University of Bern, Bern, Switzerland.
Ronald Dijkman *Institute for Infectious Diseases, University of Bern, Friedbühlstrasse 25, Bern, CH-3001, Switzerland. ronald.dijkman@unibe.ch.

Funding

ICRAD 2821ERA24Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung IZCOZ0_220329
6 · The paper itself

Abstract

Whole-genome sequencing (WGS) is essential for monitoring the genetic diversity of influenza A virus (IAV) across host species. We optimized a multisegment RT-PCR (mRT-PCR) protocol to enhance amplification of all eight IAV segments using modified RT and PCR conditions. Additionally, we introduced a dual-barcoding approach for the Oxford Nanopore platform, enabling high-throughput multiplexing without compromising sensitivity. The resulting workflow is robust, scalable, and effective for avian, swine, and human IAV samples, even at low viral loads. This approach strengthens genomic surveillance at the human-animal interface, supporting early detection, evolutionary monitoring, and rapid identification of IAV spillover events.

Indexed as

Genome, ViralHigh-Throughput Nucleotide SequencingInfluenza A virusWhole Genome SequencingAnimalsBirdsHumansInfluenza, HumanSwineWorkflowAvian IAVHigh-throughputHuman IAVInfluenza A virusOxford Nanopore sequencingSwine IAVWhole-genome sequencing

Identifiers

PMID41013580
PMCPMC12465963

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.