ArticleStem cell research & therapy2025
Wound healing effects of exosomes from hypoxia- and normoxia-expanded adipose-derived stem cells of infant and adult origin.
Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- A bioadhesive antioxidant dual-crosslinked hydrogel mitigates endothelial pyroptosis to enhance ischemic flap survival.Journal of nanobiotechnology · 2026Article
- Mechanism of saffron extract in promoting burn wound healing by modulating the Nrf2/HO-1/NLRP3 cascade to promote macrophage M2 polarization.Journal of molecular histology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundExosomes derived from adipose-derived stem cells (ADSC-Exos) have emerged as promising therapeutic agents for promoting wound healing through paracrine mechanisms. While adult ADSC-Exos have been extensively studied, those derived from infant ADSCs have received limited attention, despite their potentially superior regenerative capacity. In addition, hypoxia preconditioning has been shown to enhance the therapeutic efficacy of stem cells and their secretome; however, its influence on exosome function across different donor ages remains unclear. This study aimed to evaluate and compare the wound healing effects of ADSC-Exos from adult and infant sources expanded under hypoxic and normoxic conditions in a diabetic mouse model.
methodsADSC-Exos were isolated from adult and infant donors cultured under normoxic and hypoxic conditions. Exosomes were characterized by morphology, transmission electron microscopy (TEM) imaging, nanoparticle tracking analysis (NTA), and Western blotting. RNA sequencing was performed to profile miRNAs encapsulated in the exosomes. In vitro assays assessed the effects of exosomes on proliferation, migration, and wound healing in human dermal fibroblasts (HDFs) under high-glucose conditions. In vivo efficacy was evaluated using full-thickness wounds in db/db diabetic mice to assess the therapeutic effects of the exosomes.
resultsNormoxic infant ADSC-Exos exhibited a broader size distribution and a larger mean particle size. Several wound-healing-related miRNAs were upregulated in hypoxic ADSC-Exos from both adult and infant sources. Normoxic infant ADSC-Exos significantly enhanced HDF proliferation and migration, whereas hypoxic adult ADSC-Exos exhibited superior early wound closure. In vivo, normoxic adult ADSC-Exos achieved the fastest wound closure at day 7, and normoxic infant ADSC-Exos showed significantly greater wound closure by day 10. All ADSC-Exos promoted dermal regeneration, with hypoxic adult ADSC-Exos showing the most pronounced effects.
conclusionInfant ADSC-Exos demonstrated inherent advantages under normoxic conditions, including enhanced effects on cell proliferation, migration and wound regeneration. While hypoxic preconditioning enhanced the efficacy of adult ADSC-Exos, it provided limited additional benefit to infant-derived exosomes, highlighting their intrinsic suitability for regenerative therapies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.