ArticleBMC genomics2025
Genomic analyses of Purpureocillium lilacinum reveal unique DNA regions for developing isolate-specific molecular markers.
Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
backgroundThe Purpureocillium lilacinum NCHU-NPUST-175 (Pl-NCHU-NPUST-175) strain has been previously reported as an entomopathogenic fungus (EPF) with potential for controlling Forcipomyia taiwana (little black mosquito). In this study, genome sequencing of Pl-NCHU-NPUST-175 was performed via long-read sequencing (Oxford Nanopore Technologies, ONT). The comparative genomic analysis was conducted to identify isolate- and species-specific DNA regions for developing markers for rapid molecular identification, in order to detect the fungus in an environment and to confirm species identity before practical field applications.
resultsThe genome assembly of Pl-NCHU-NPUST-175 is 36.55 Mb, consisting of 13 contigs with a GC content of 58.64%. The largest contig of the Pl-NCHU-NPUST-175 genome was 6.79 Mb, and the N50 value was 4.08 Mb. The genome contains 14,069 putative protein-coding genes, with a gene density of 384.90 genes per Mb and a median number of three exons per gene. Additionally, one mitogenome, Pl-NCHU-NPUST-175, was assembled with a size of 0.23 Mb and contained 16 protein-coding genes. In the Pl-NCHU-NPUST-175 genome, repeat regions accounted for 3.70% of the genome. Most repetitive sequences exhibit a nucleotide sequence divergence of less than 30%. Phylogenetic analysis revealed that Pl-NCHU-NPUST-175 is most closely related to other P. lilacinum strains. To identify distinctive genomic regions (DGRs), a comparative genomics analysis was conducted between Pl-NCHU-NPUST-175, P. takamizusanense and P. lilacinum. A total of 1,154 unaligned fragments were identified by genome-wide alignment, ten DGRs were randomly selected for specific primer design, and the specificity of the primer sets was tested. The results showed that all primer sets could serve as specific molecular markers for Pl-NCHU-NPUST-175. Among the ten primer sets, three primer sets were selected for the soil-sample detection test. The qualitative PCR results indicate that all three isolate-specific primer sets (pl_1, pl_8, and pl_10) detected 10
conclusionsThe genome sequence of Pl-NCHU-NPUST-175 was decoded and compared with those of closely related isolates and species. Based on a developed comparative genomics approach, we highlight the feasibility of developing specific molecular markers of EPF, and this method could be further applied to commercialize EPF products to address sustainability in the natural environment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.