ArticleSensors (Basel, Switzerland)2025
Sequential Injection Analysis of Cholesterol Using an Oxidation-Reduction Electrode Detector.
Article in Sensors (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A new automated method for the determination of cholesterol in serum was developed by combining sequential injection analysis (SIA) with potentiometric detection using a gold oxidation-reduction potential (ORP) electrode because serum cholesterol is an important indicator of abnormal lipid metabolism, arteriosclerosis, and hypertension in clinical diagnosis. The method is based on enzymatic hydrolysis of cholesterol esters by cholesterol esterase (CE) to yield free cholesterol, followed by oxidation with cholesterol oxidase (COD) to produce hydrogen peroxide. In the presence of horseradish peroxidase (HRP) and potassium ferrocyanide (K
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